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| 塞来昔布对胃癌细胞增殖的影响 | |
| 引用本文： | 凌桂琴,王绪,王琳,李小翠. 塞来昔布对胃癌细胞增殖的影响[J]. 河南医学研究, 2006, 15(4): 332-335 |
| 作者姓名： | 凌桂琴 王绪 王琳 李小翠 |
| 作者单位： | 1. 江苏省徐州医学院第二附属医院肿瘤内科,江苏徐州,221006 2. 徐州医学院医学影像学系,江苏徐州221006 3. 徐州医学院组胚教研室,江苏徐州221006 4. 徐州医学院微免教研室,江苏徐州,221006 |
| 摘 要： | 目的:研究选择性环氧合酶-2(Cyclooxygenase-2,COX-2)抑制剂塞来昔布(celecoxib)对人胃癌细胞株SGC7901增殖的影响及可能作用机制。方法:采用MTT法检测细胞增殖,放免法检测SGC7901细胞6-酮-PGF1α的含量,免疫细胞化学染色法检测SGC7901细胞VEGF的表达。结果:Celecoxib可明显抑制SGC7901细胞的增殖,以及抑制细胞6-酮-PGF1α的释放和VEGF的表达。结论:Celecoxib可明显抑制胃癌细胞SGC7901的生长,其可能的作用机制是抑制细胞6-酮-PGF1α的释放和VEGF的表达。 |
| 关 键 词： | 胃癌 塞来昔布(celecoxib) 增殖 VEGF 6-酮-PGF_(1α) |
| 文章编号： | 1004-437X（2006）04-0332-04 |
| 收稿时间： | 2006-08-01 |
| 修稿时间： | 2006-12-23 |
Influence of celecoxib on proliferation of human gastric cancer cell line |
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| LING Gui-qin,WANG Xu,WANG Lin,LI Xiao-cui. Influence of celecoxib on proliferation of human gastric cancer cell line[J]. Henan Medical Research, 2006, 15(4): 332-335 | |
| Authors: | LING Gui-qin WANG Xu WANG Lin LI Xiao-cui |
| Affiliation: | 1. Department of Ontology, The Second Affdiated Hospital of Xuzhou Medical College, Xuzhou 221006, China ; 2. Department of Microbiology , Xuzhou Medical College, Xuzhou 221006, China; 3. Department of Histology and Embryolog , Xuzhou Medical College, Xuzhou 221006, China ; 4. Departmant of Radiology, Xuzhou Medical College, Xuzhou 221006, China |
| Abstract: | Objective:To evaluate the effects and mechanisms of celecoxib on proliferation of human gastric cancer cells line.Methods: The proliferration of gastric cancer cell line was measured by using MTT assay.radioimmunoassay(RIA) was used to determine the influence of celexoxib on the level of 6-keto-PGF_(1α) in the supenatant of cultured gastric cancer cell.The effect of celecoxib on expression of VEGF was analyzed by using immunocellular chemical staining.Results: The growth of human gastric cancer cell line SGC7901 was inhibited by celecoxib.Treated with celecoxib,the production of 6-keto-PGF_(1α) in the supernatant of cultured cell was suppressed in a dose-dependent manner.Celecoxib also decreased the expression of VEGF and in a time-dependent manner.Conclusion: Celecoxib inhibites the growth of gastric cancer cell via suppression of 6-keto-PGF_(1α) and VEGF. |
| Keywords: | VEGF |
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