超抗原SEA活化淋巴细胞抗肿瘤活性的实验研究

纯化SEA在 10 -12 ～ 10 -5g/ml浓度范围对体外培养的BALB/c鼠脾细胞表现了细胞增殖诱导能力 ,并呈剂量依赖关系 ,其中 10 -7～ 10 -5g/mlSEA的作用强于最适量 ( 2 5 μg/ml)PHA。在E/T为 5∶1～ 2 0∶1条件下 ,10 -5g/mlSEA活化 48h的BALB/c鼠脾细胞对YAC 1细胞的杀伤活性高于NK细胞 ,但SEA未能增强BALB/c鼠脾细胞对B16细胞的杀伤活性。 5 μg和5 0 μgSEA体内用药对L615白血病无治疗作用 ,但转输经SEA活化的同系小鼠脾细胞对L615白血病有一定疗效。实验结果提示 ,SEA活化淋巴细胞对NK敏感的淋巴瘤、白血病有杀伤及治疗作用 ,这种作用依赖于较大数量级SEA活化淋巴细胞的存在 ,并且与SEA活化T细胞分泌的细胞因子有关

The Anti-tumor Activities of Superantigen SEA on the Activation of Lymphocytes

The purified SEA could induce the proliferation of in vitro cultivated splenocytes of BALB/c mice in a dosedependent manner in which 10 -7 ～10 -5 g/ml of SEA showed a stronger activity than the optimal dose of PHA (25μg/ml) ,and under a condition of E/T at 5:1～20∶1,the killing activities of the 48 hours cultivated splenocytes of BALB/c mice activated by 10 -5 g/ml of SEA on the YAC1 cells were even stronger than those of NK cells However,SEA was unable to promote the killing activities of splenocytes of BALB/c mice on B16 cells,and 5μg and 50μg of SEA had no therapeutical effect on L 615 leukemia cells;but the transfer of SEAactivated syngeneic splenocytes of mice showed certain effects on L 615 leukemia cells These experimental results suggest that SEAactivated lymphocytes demonstrate certain killing and therapeutical effects on the NK cellsensitive lymphoma and leukemia cells These effects depend upon the existence of activated lymphocytes in greater amount and relate to the cytokines secreted by activated T cells