|
|||||
|
|
| 电针治疗大鼠神经痛后脑内孤啡肽受体mRNA表达的变化 | |
| 引用本文： | 马飞,谢虹,董志强,李为民,王彦青,吴根诚.电针治疗大鼠神经痛后脑内孤啡肽受体mRNA表达的变化[J].上海针灸杂志,2004,23(4):32-35. |
| 作者姓名： | 马飞 谢虹 董志强 李为民 王彦青 吴根诚 |
| 作者单位： | 1. 复旦大学上海医学院中西医结合系,针刺原理研究所,上海,200032;新乡医学院生理教研室,河南,453003 2. 复旦大学上海医学院中西医结合系,针刺原理研究所,上海,200032;天津医科大学总医院麻醉科,天津,300052 3. 复旦大学上海医学院中西医结合系,针刺原理研究所,上海,200032 |
| 基金项目： | 国家自然科学基金资助 (3 0 0 70 948) |
| 摘 要： | 目的 观察电针治疗大鼠神经痛前后脑内一些与痛觉相关的核团孤啡肽受体mRNA表达的变化。方法 实验分为3组，对照组为正常大鼠，神经痛组为大鼠坐骨神经慢性限制性损伤致神经痛模型，电针组为术后第7日给予神经痛大鼠电针治疗30min。各组动物处死后，取脑组织，冰冻切片，采用原位杂交的方法。观察电针后脑内与痛觉相关的中脑导水管周围灰质腹外侧(vIPAG)、中缝背核(DRN)、中缝大核(NRM)核团孤啡肽受体mRNA表达的变化。结果 大鼠坐骨神经结扎后出现痛敏，电针能明显抑制大鼠痛敏；神经痛大鼠给予电针后脑内vIPAG、DRN、NRM中孤啡肽受体mRNA表达明显降低。结论 实验提示脑内孤啡肽受体可能参与电针镇痛过程。 |
| 关 键 词： | 电针 大鼠 神经痛 孤啡肽受体 镇痛 阿片受体 |
| 文章编号： | 1005-0957(2004)04-0032-04 |
| 修稿时间： | 2003年12月13 |
Effect of Electroacupuncture on Expression of ORL1 Receptor mRNA in Some Brain Nuclei of the Neuropathic Pain Rats |
|
| MA Fei ,XIE Hong ,DONG Zhi qiang ,LI Wei min ,WANG Yan qing ,WU Gen cheng.Effect of Electroacupuncture on Expression of ORL1 Receptor mRNA in Some Brain Nuclei of the Neuropathic Pain Rats[J].Shanghai Journal of Acupuncture and Moxibustion,2004,23(4):32-35. | |
| Authors: | MA Fei XIE Hong DONG Zhi qiang LI Wei min WANG Yan qing WU Gen cheng |
| Institution: | MA Fei 1,2,XIE Hong 1,3,DONG Zhi qiang 1,LI Wei min 1,WANG Yan qing 1,WU Gen cheng 1 1. Department of Integrative Medicine,Institute of Acupuncture Research,Shanghai Medical College,Fudan University,Shanghai 200032,China, 2. Department of Physiology,Xinxiang Medical College,Henan 453003,China, 3. Department of Anesthesia,the General Hospital of Tianjin Medical University,Tianjin 300052,China |
| Abstract: | Objective To investigate the effect of electroacupuncture (EA) on the expression of ORL 1 receptor mRNA in some brain nuclei of the neuropathic pain rats. Methods Rats were divided into three groups: control group, neuropathic pain group and EA group. Rats were given four loose ligation to sciatic nerve. On day 7 after operation, rats with hyperalgesia to radiant heat were selected as neuropathic pain rats and one group was administered with EA for 30 min. Expression of ORL 1 receptor mRNA in nucleus raphe magnus (NRM), ventrolateral periaqueductal gray (vlPAG) and dorsal raphe nucleus (DRN) of the neuropathic pain rats was investigated using in situ hybridization technique. Results The results showed that hyperalgesia appeared after sciatic nerve chronic constriction injury to rats and EA could depress the hyperalgesia. A significant decrease in ORL 1 receptor mRNA expression was observed in the nuclei of vlPAG, DRN, NRM after EA. Conclusion Our results suggested that ORL 1 receptor was involved in EA analgesia. |
| Keywords: | Orphanin FQ ORL 1 receptor Neuropathic pain EA analgesia |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |