Inhibition of long-distance movement of RNA silencing signals in Nicotiana benthamiana by Apple chlorotic leaf spot virus 50 kDa movement protein |
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Authors: | Yaegashi Hajime Tamura Akihiro Isogai Masamichi Yoshikawa Nobuyuki |
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Affiliation: | a The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, Japan b Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan |
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Abstract: | Apple chlorotic leaf spot virus 50 kDa movement protein (P50) acts as a suppressor of systemic silencing in Nicotiana benthamiana. Here, we investigate the mode of action of P50 suppressor. An agroinfiltration assay in GFP-expressing N. benthamiana line16c (GFP-plant) showed that P50 could not prevent the short-distance spread of silencing. In grafting experiments, the systemic silencing was inhibited in GFP-plants (scion) grafted on P50-expressing N. benthamiana (P50-plant; rootstock) when GFP silencing was induced in rootstock. In double-grafted plants, GFP-plant (scion)/P50-plant (interstock)/GFP-plant (rootstock), the systemic silencing in scion was inhibited when GFP silencing was induced in rootstock. Analysis of P50 deletion mutants indicated that the N-terminal region (amino acids 1-284) is important for its suppressor activity. In gel mobility shift assay, P50 lacks binding ability with siRNAs. These results indicated that P50 has a unique suppressor activity that specifically inhibits the long-distance movement of silencing signals. |
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Keywords: | ACLSV RNA silencing suppressor Movement protein Systemic silencing Silencing signal Long-distance movement |
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