胆碱酯酶生物传感器检测农药残留物研究进展

旨在综述近年来以胆碱酯酶(ChE)作为生物识别元件的有机磷农药生物传感器的研究进展;评述了这类传感器在专一性、精确性、基体效应和酶的不可逆抑制等方面存在的问题,以及由此研究的一些新型检测方法;指出了设计灵敏的ChE突变体作为生物识别元件,以及开发多样品和多组分平行分析系统是这类生物传感器在商业化过程中的关键课题。

Study on characteristic of biosensors associated with cholinesterase for detection of pesticide residues

Current research activity in characteristic of biosensors associated with cholinesterase (ChE) for detection of organophosphate and carbamate pesticide residues is summarized. Some novel methods, which aimed to overcome the problems inherent to ChE inhibition analysis, namely selectivity, accuracy, irreversible inhibition and matrix effect, etc, are commented. More sensitive genetically engineered ChE variants and sophisticated parallel detection systems are also envisaged when it comes to turning biosensors based on ChE into marketable products, respectively.