ELISA联合检测方法的建立及应用

目的在微孔板平台上建立多指标联合检测的方法,为急诊、门诊及基层等多种医疗机构提供1种新的血清学检测手段。方法本研究根据酶联免疫吸附法(ELISA法)原理,以卵泡刺激素(FSH)、泌乳素(PRL)、黄体生成素(LH)、生长激素(GH)为检测靶标,在自行设计的聚苯乙烯板孔上完成反应体系的优化,包括FSH、PRL、LH、GH包被抗体浓度及酶标抗体浓度,建立1种并行分析样品中多种指标的方法。结果成功确定4种垂体激素联合检测的条件,包括4种激素抗体包被浓度、酶标抗体浓度等,并成功应用于临床样本的检测。结论本研究首次建立了1种简单、特异性高、成本低、并行分析4种垂体激素的联合检测方法,提高了常规ELISA法的检测效率。

Establishment and preliminary application of ELISA joint detection

Objective Multi‐index method of establishing joint detection on a microplate platform to provide a new measure of serological tests for the emergency room ,outpatient and primary and other medical institutions .Meth‐ods This study was based on the principle of ELISA with FSH ,PRL ,LH and GH for the detection of target to com‐plete the optimization of the reaction system in polystyrene plate hole own design ,including FSH ,PRL ,LH ,GH coa‐ted antibody concentration and enzyme labeled antibody concentration ,and establish a method for simultaneous analy‐sis of multiple indicators in a sample .Results The conditions of four kinds of pituitary hormones combined detection has been determined successfully ,including that four kinds of hormone antibody peridium concentration ,enzyme la‐beled antibody concentration ,and detection and successfully applied in clinical samples .Conclusion This study is the first to establish a simple ,high specificity ,low cost ,parallel analysis of the combined pituitary four detection methods to improve the detection of conventional ELISA method efficiently .