孕酮对人T淋巴细胞体外活化CD69表达的作用

目的：探讨孕酮（Ｐｒｏｇｅｓｔｅｒｏｎｅ，Ｐｒｏｇ）及地塞米松（Ｄｅｘａｍｅｔｈａｓｏｎｅ，Ｄｅｘ）对人外周血Ｔ淋巴细胞体外活化ＣＤ６９表达的作用。方法：以女性健康志愿者（１０名）为研究对象，以蛋白激酶Ｃ的刺激剂佛波醇酯（Ｐｈｏｒｂｏｌ ｅｓｔｅｒ，ＰＤＢ）为Ｔ淋巴细胞的活化剂，采用双荧光染色流式细胞技术。检测ＣＤ３＾＋的Ｔ淋巴细胞表达早期活化表面分子ＣＤ６９的百分率，以观察Ｐｒｏｇ、Ｄｅｘ及Ｐｒｏ０ｇ加上Ｄｅｘ对外周血Ｔ淋巴细胞体外活化的作用。结果：在体外培养条件下，无论单独使用Ｐｒｏｇ还是Ｄｅｘ均地强低剂量ＰＤＢ刺激ＣＤ６９的表达。然而，与单独使用Ｐｒｏｇ或Ｄｅｘ的作用相比。Ｐｒｏｇ加上Ｄｅｘ明显减弱低剂量ＰＤＢ的活化ＣＤ６９的表达。结论：同时使用Ｐｒｏｇ与Ｄｅｘ可明显抑制Ｔ淋巴细胞的体外活化，提示在临床上

Effect of progesterone on expression of CD69 by human in vitro activated T lymphocytes

Objective:An investigation was performed to study effect of progesterone ( Prog ) and dexamethasone (Dex) on the expression of CD69 by activated T lymphocytes of human peripheral blood Methods:Studies were performed on 10 healthy female volunteers Phorbol ester (PDB), a stimulator of Protein kinase C, was used as an activator of T lymphocytes The techniques of two color immunofluorescence staining and flow cytometry were applied to the evaluation of expression rate of CD69, a surface marker expressed in early phase of T lymphocyte activation, by CD3 population in order to examine effect of Prog, Dex and Prog plus Dex on activation of T lymphocytes Results:Either Prog or Dex enhanced CD69 expression by the T lymphocytes in vitro stimulated with low dosage of PDB, whereas simultaneous addition of Prog and Dex reduced significantly CD69 expression as compared to the expression rate in the presence of Prog or Dex alone Conclusion:Simultaneous application of Prog and Dex can significantly inhibit activation of T lymphocytes This results indicate the combinational treatment of Prog and Dex may would produce inhibitory effect on immuno inflammation in clinical therapy of autoimmune diseases