| 脊髓挤压伤后BDNF mRNA表达的RT-PCR研究 |
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| 引用本文： | 柏顺明,冯忠堂,李明,陈彦红,王廷华. 脊髓挤压伤后BDNF mRNA表达的RT-PCR研究[J]. 昆明医学院学报, 2002, 23(3): 14-17 |
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| 作者姓名： | 柏顺明 冯忠堂 李明 陈彦红 王廷华 |
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| 作者单位： | 1. 昆明医学院神经科学研究所,昆明,650031;昆明医学院第一附属医院神经外科,昆明,650032
2. 昆明医学院神经科学研究所,昆明,650031 |
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| 基金项目： | 云南省自然科学基金资助项目(NO.2000C0057M);云南省教委基金资助项目(NO.0012012) |
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| 摘 要： | 目的探讨实验性脊髓挤压伤后内源性神经营养素BDNF mRNA在不同时段的表达变化.方法采用成年SD大鼠简易脊髓挤压伤模型.一步法抽提脊髓组织总RNA,分光光度计测OD260,OD280值.β-actin作内参照,RT-PCR技术检测内源性神经营养素BDNF mRNA在不同时段的表达.结果(1)用RT-PCR技术检测到BDNF mRNA在正常大鼠脊髓表达.(2)脊髓挤压伤后BDNF mRNA在不同时段表达变化趋势不一.术后24h组、5d组、21d组BDNF mRNA的表达水平较正常组显著增高(P<0.05),而术后14d组虽高于正常组水平,但与正常组及损伤后除24h组外各组相比均无显著性差异(P>0.05).此外,除伤后24h组与14d组,伤后各组间比较亦均无显著性差异,表明脊髓挤压伤后24h,5d BDNF-mRNA表达明显增加,而14d略有下降,21d又有回升.结论(1)在正常大鼠脊髓有BDNF mRNA表达,提示BDNF可能与脊髓的生理功能有关.(2)cSCI后24h,5d,21d BDNF mRNA表达增高,可能有利于BDNF的合成,进而BDNF可能参与神经损伤修复.(3)BDNF mRNA于术后24h,5d明显升高,14d又回复近正常水平,而21d又明显回升,表明BDNF的表达在挤压伤脊髓内经历了双向变化.
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| 关 键 词： | 脊髓挤压伤 BDNF mRNA RT－PCR 研究 |
| 文章编号： | 1003-4706(2002)03-0014-04 |
| 修稿时间： | 2002-05-15 |
The Change of Expression on the BDNF mRNA in Spinal Cord Following cSCI |
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| BAI Shun-ming ,),FENG Zhong-tang ,),LI Ming ),CHEN Yan-hong ),WANG Ting-hua ). The Change of Expression on the BDNF mRNA in Spinal Cord Following cSCI[J]. Journal of Kunming Medical College, 2002, 23(3): 14-17 |
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| Authors: | BAI Shun-ming ) FENG Zhong-tang ) LI Ming ) CHEN Yan-hong ) WANG Ting-hua ) |
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| Affiliation: | BAI Shun-ming 1,2),FENG Zhong-tang 1,2),LI Ming 2),CHEN Yan-hong 2),WANG Ting-hua 2) |
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| Abstract: | Objective: To explore the change of expression of the BDNF-mRNA in spinal cord in different time following cSCI. Methods: The spinal cord of adult srague-daweley rats at the T13 and L 1 levels was crushed to establish crushed spinal cord model. Total RNA was extracted with TRIzolR reagent, and BDNF gene was amplified by RT-PCR technique. Results: (1) BDNF mRNA is observed in spinal cord of normal animals by RT-PCR. (2) BDNF mRNA is upregulated in spinal cord on 24?h, 5?d, 21?d following SCI(P>0.05), while the level of BDNF mRNA on 14d following SCI keeps no difference compared with control group. The results indicates that the BDNF mRNA expression on 24?h,5?d,21?d following cSCI is different from that seen on 14?d postlesion. Conclusion: The BDNF mRNA is expressed in spinal cord of normal rats and the expression of BDNF mRNA is different in different time following cSCI. The change of BDNF mRNA suggests that BDNF plays an important role in traumatic spinal cord. |
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| Keywords: | Spinal cord injury BDNF mRNA RT-PCR Expression |
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