腺病毒介导多基因对大鼠脾淋巴细胞毒作用的影响

目的 研究含多基因（p53、GM-GSF、B7-1、IL-2）的重组腺病毒载体Ad-multigenes，对大鼠脾脏淋巴细胞毒作用的影响及对淋巴细胞分泌IL-2的刺激作用。方法 应用人外周血淋巴细胞和肿瘤细胞混合培养，分析导入目的基因的肝癌细胞系体外刺激人T淋巴细胞分泌IL-2的作用；利用大鼠脾淋巴细胞杀伤活性试验，分析导入目的基因的大鼠癌肉瘤Walker256细胞，其名疫原性的变化。结果 导入Ad-multigenes的肝癌细胞系体外刺激人外周血T淋巴细胞分泌IL-2的水平增加，导入Ad-multigenes的大鼠Walker256细胞，能增加大鼠脾脏淋巴细胞的杀辛本瘤细胞活性。结论 腺病毒介导多基因Ad-multigenes、能增加大鼠癌肉瘤Walk-er256细胞的免疫原性，和T细胞分泌IL-2的水平增加。

Effect of adenovirus-mediated multigenes on cytotoxicity of rat spleen lymphocyte in vitro

Objective To investigate the changes of the cytotoxicity of rat spleen lymphocyte and the level of IL 2 secreted by human T lymphocyte after the induction of adenovirus mediated multigenes (Ad multigenes, containing p53, GM CSF, B7 1, IL 2 genes). Methods After human lymphocytes of peripheral blood and tumor cells were cultured together, the level of IL 2 secreted by T lymphocytes was determined after they were stimulated by liver cancer cells with pre transfer of Ad multigenes in vitro by ELISA. The change of the immunogenicity of rat carcinosarcoma cell Walker 256 transduced with multigenes was studied by cytotoxicity assay of rat spleen lymphocytes. Results The level of IL 2 secreted by peripheral blood T lymphocytes was increased in vitro after the T cells were co cultivated with Ad multigene transducted liver cancer cells. Stimulated by Ad multigene transducted Walker 256 cells, the cytotoxicity activities of rat spleen lymphocyte were significantly elevated. Conclusion The immunogenicity of rat carcinosarcoma cell Walker 256 is enhanced, and the IL 2 production level which was secreted by T lymphocyte is increased after the mediation of Ad multigenes.