三氧化二砷对人宫颈癌细胞Fas 表达和钙含量的影响

 目的 研究人宫颈癌Hela细胞经三氧化二砷(As₂O₃)处理后的Fas表达和钙含量变化。方法利用MTT法观察As₂O₃对Hela细胞的生长抑制作用，Annexin V-FITC+PI双参数检测细胞凋亡。流式细胞仪测定As₂O₃处理后Hela细胞Fas阳性百分率及胞内钙离子(IECa²⁺)含量变化。结果 (1) As₂O₃可显著抑制Hela细胞生长，且剂量-效应和时间-效应关系显著(P<0．05)，其24h、48h和72h的IG50值分别为8．62μmol／L、6．77μmol／L和4．89μmol／L。(2) As₂O₃处理后Hela细胞凋亡率明显增加。(3) As₂O₃能显著增加Fas蛋白表达和IECa²⁺含量(P<0．01)。结论 As₂O₃在体外可显著抑制Hela细胞生长并引起凋亡，其机制可能与上调Fas表达和IECa²⁺含量有关。

Change of Fas Expression and Intracellular Calcium Content in Human Cervical Carcinoma Cells Treated with Arsenic Trioxide

Objective 　The study was to research Fas expression and int racellular calcium content change in human cervical carcinoma Hela cells t reated with arsenic t rioxide. Methods 　MTT method was used to observe the growth inhibition. Apoptosis was detected with Annexin V-FITC + PI dual parameter. Fas positive rate and int racellular calcium ions ( IECa ²⁺ ) levels were measured by flow cytometry in Hela cells treated with As₂O₃ . Results 　As₂O₃ could inhibit the growth of Hela cells dramatically. There was obvious dosage-effect and time-effect correlations , it s half inhibitory concent ration ( IC50 ) at 24h , 48h and 72h were 8. 62μmol/ L , 6. 77μmol/ L and 4. 89μmol/ L respectively. Apoptosis rate increased in Hela cellst reated with As₂O₃ . As₂O₃ could increase Fas expression and IECa ²⁺ levels in Hela cells ( P < 0. 01) . Conclusion 　As₂O₃ could inhibit the growth of Hela cells dramatically and induce apoptosis , we proposed that its mechanisms were probably associated with the improved Fas expression and IECa ²⁺ levels.