阳离子脂质体介导HSV-TK基因对人脑胶质瘤细胞的杀伤作用

目的 观察阳离子脂质体介导的HSV－TK／ACV系统对人脑胶质瘤细胞的杀伤作用。方法 将新近克隆的含HSV－TK基因的真核表达载体pCR3-5K,用阳离子脂质体Lipofectamine转染至人脑胶质瘤细胞株TJ905中，筛选出阳性克隆，给予不同浓度的ACV，观察不同时间对细胞的杀伤情况，并用MTT方法检测不同细胞对ACV的反应情况。结果 转染TK基因的胶质瘤细胞对ACV的杀伤敏感性明显提高（P＜0．01），ACV对转染TK基因的胶质瘤细胞有特异性抑制和杀伤作用。结论 阳离子脂质体Lipofectamine是一种简便、安全、有效的基因转移方法。可用阳离子脂质体介导pCR3-TK转移，进行胶质瘤HSV－TK／GCV基因治疗的研究，为胶质瘤的治疗提供一种新方法。

Killing effects of HSV-TK gene on human glioma mediated by cationic liposome

Objective To observe HSV TK/ACV system killing human glioma transferred with cationic liposome.Methods The eukaryotic expressing vectors pCR3 TK cloned containing the HSV TK gene were transferred into TJ905 cells with cationic liposome,Lipofectamine.After transfection,G418 was used to select the positive clones.The killing of TJ905 glioma cells transfected with HSV TK was examined by MTT under various concentration of ACV.Results The increasing sensitivity level of the TK gene transferred glioma cell to ACV treatment was confirmed.ACV had a specific suppression and killing effects on the glioma cells transfected with TK gene.Conclusion Cationic liposome,Lipofectamine is safe,simple and effective in transferring gene.This indicates that HSV TK/ACV gene therapy of glioma may be studied by cationic liposome transferring PCR3 TK.Such treatment may be used as an innovative method for brain tumor therapy.