人胚胎小脑突触体素及NOS阳性细胞的观察

目的 研究人体胚胎时期小脑中突触体素及ＮＯＳ阳性细胞的组织学变化。方法用免疫组织化学、组织化学技术，对４￣９个月胎儿小脑皮质突触体纱和ＮＯＳ阳性细胞进行了观察。结果 突触体素在小脑不同层中的分布各不相同，外颗粒层４￣９个月均无阳性颗粒出现。４个月时除粒层各层免疫反应颗粒均很少，５个月时中间层的以较各层深，６个月时分子层的颗粒沿Ｂｅｒｇｍａｎｎ纤维方向分布，７个月时分子层染色颗粒呈明显的网状分布。中

OBSERVATION ON SYNAPTOPHYSIN AND NOS POSITIVE CELLS OF HUMAN FETUS CEREBELLUM

ObjectiveSynaptophysin and NOS positive cells were studied histologically in the cerebellum of different periods of human fetus.MethodsBy immunohistochemistry and histochemistry technique,the synaptophysin and NOS positive cells of 4-9 month human fetus cerebellar cortex were observed.ResultsDistribution of synaptophysin was different in different layer of the cerebellum.In 4-9 months,the external granular layer was unstained.In 4 months,immunoreactive granules in all layers except external granular layer were scarce.In 5 months,stain of the middle layer was stronger than the other layers.In 6 months,the punctate immunoreactive product of the middle layer was distributed through the Bergmann filament ,stain of the middle layer was stronger than 5 months.In 7 months,the reactive product in the middle layer was distributed like a network,the punctate product of the middle layer and the internal granular layer was denser than 6 months.In 8 months,the profile of the Purkinje cell could be found,positive punctate products outlined the main dendrite of the Purkinje cell.In 4-6 months,no NOS positive cells were found in the cerebellar cortex,but the capillary plexus was stained .In 7-8 months,dense positive NOS cells were distributed in the cerebellar cortex,but the stain was shallow,the delimites of the cells were not clear.ConclusionThe amount of synaptophysin could reflect developing degree of the synapes in the cerebellum,synaptophysin increased with the development of the neuron cells ,and the increase was predominant in the molecular layer in 7-8 months.