IL—2,IL—6对地塞米松诱导小鼠胸腺细胞凋亡的调节作用

目的：以Ｄｅｘ诱导小鼠胸腺细胞凋亡为模型，研究细胞因子（ＩＬ－２、ＩＬ－６）对小鼠胸腺细胞凋亡的调节作用。方法：应用ＰＩ法检测亚二倍体细胞，二苯胺法测定胸腺细胞片段化ＤＮＡ含量（％），ＤＮＡ凝胶电泳，流式细胞计分析胸腺细胞表型、测定高钙细胞百分比。结果：发现地塞米松（Ｄｅｘ）与小鼠胸腺细胞共同培养引起细胞凋亡，胸腺细胞减少以ＣＤ４^ ＣＤ８^ 细胞最明显。细胞凋亡具有时间效应并与Ｄｅｘ浓度有关。３～４

The regulation ofcytokines on apoptosis induced by dexamethasone in mouse thymocyte

Objective: IL2 and IL6 regulated the apoptosis of murine thymocytes induced by Dex .Methods: PI staining,DNA fragmentation,DNA gel electrophoresis,FACScan,and so on. Results: Apoptosis induced by Dex in mouse thymocytes has been shown that the number of thymocytes was decreased,especially CD4 CD8 cells.The apoptosis of thymocytes depends on the incubation time and the dose of Dex.The thymocytes from 24 weeks old mice are more sensitive to Dex than those from 68 weeks old mice. It has been shown show that high dose IL2(>200 U/ml) or low dose IL2(100 U/ml) with IL6(100 U/ml) could reduce the DNA fragmentation and the percentage of apoptotic mucleic (hypodiploid cells) in the thymocytes treated with Dex.Cytosolic calcium ion concentration and distribution of subpopulation of mouse thymocytes are corrected by these cytokines.IL6 alone has no effect on this apoptosis. Conclusion: Dex could induce the apoptosis of murine thymocytes,which could be reduced by high dose IL2(>200 U/ml) or low dose IL2(100 U/