IL-2促进大鼠子宫平滑肌细胞增殖及IL-2R的表达

目的：研究白细胞介素-2(IL-2)对大鼠子宫平滑肌细胞增殖的影响，并探讨其可能的机制。方法：以培养的大鼠子宫平滑肌细胞进行了以下实验：以MTT比色法，测定IL-2对该细胞增殖的剂量效应；用流式细胞技术，观察IL-2对该细胞细胞周期各阶段DNA含量的影响；用激光共聚焦显微镜技术，检测IL-2对该细胞内Ca^2 水平的影响；用RT-PCR技术，检测IL-2R在该细胞的表达。结果：IL-2(10～1000U／ml)可显著促进大鼠子宫平滑肌细胞的增殖。用流式细胞技术检测细胞中DNA的含量发现，IL-2(100 U／ml)可提高该细胞由G1期进入S期的比例。激光共聚焦法检测到IL-2(100U／ml)可升高细胞内的Ca^2 浓度。RT-PCR技术检测到细胞表达IL-2R a mRNA、IL-2RβmRNA和lL-2R γmRNA三条链。结果：IL-2参与了对大鼠子宫平滑肌细胞增殖的调节，其促进增殖作用可能与该细胞周期DNA含量的变化、Ca^2 浓度的改变和IL-2R的表达有关。

Enhancement effect of interleukin-2 on the proliferation of cultured rat uterin smooth muscle cells and the expression of interleukin-2 receptors

Objective:To investigate the effect o f IL-2 on the proliferation of rat uterin smooth muscle cells and explore its possible mechanism. Methods: Enha ncement effect of IL-2 on proliferation of cultured rat uterin smooth muscle cel ls, the effect of IL-2 on DNA content at various periods of cell cycle and on Ca 2+ concentration in the cells and the expression of interleukin-2 receptor s were detected or observed by MTT colorimetry, flow cytometry, laser scanning confoca l microscope and RT-PCR, respectively. Results:I L-2 of 10 to 1 000 U/ml signific antly stimulated the proliferation of cultured rat uterin smooth muscle cells. I L-2(100 U/ml) increased the ratio of rat uterin smooth muscle cells in G 1 and S phas es and increased the Ca 2+ release from rat uterin smooth muscle cells. Conclusion:IL-2 significantly enhanced the proliferation of rat uterin smooth muscle ce lls. The enhancement effect of IL-2 on rat uterin smooth muscle cell proliferati on is possibly related to the altering of DNA content, increased Ca 2+ conc entration and expression of interleukin-2 receptors in the cells.