降压清心方对高血压大鼠心肌纤维化及神经内分泌影响的实验研究

目的：观察降压清心方对自发性高血压大鼠（SHR）心肌纤维化及血管紧张素Ⅱ（AngⅡ）、Ⅲ型前胶原氨基端肽（PⅢNP）的影响。方法：将24只8～10周龄雄性SHR随机分为4组：模型组、中药组（降压清心方组）、西药组（雷米普利组）、中西医结合组（降压清心方＋雷米普利组），每组6只，并设正常8周龄SD大鼠6只做正常组。给药4周后检测AngⅡ、PⅢNP及病理观察。结果：连续给药4周，模型组血浆AngⅡ、PⅢNP水平均高于正常组（P〈0．01），中药组、西药组、中西结合组血浆。AngⅡ和PⅢNP含量水平低于模型组，西药组和中西结合组与模型组比较具有统计学意义（P〈0．05）；正常组心肌纤维排列整齐，心肌细胞大小、形态正常，模型组心肌细胞肥大，心肌间质胶原纤维明显增多，围绕心肌细胞的胶原纤维排列紊乱，中药组心肌间质轻度增生，心肌细胞轻-中度肥大，西药组心肌纤维排列较整齐，细胞横径略有增大，中西结合组心肌间质胶原纤维明显减少，相邻细胞的胶原纤维排列整齐。结论：降压清心方可以抑制自发性高血压大鼠心肌纤维化，其机理是通过抑制AngⅡ、PⅢNP发挥作用。

Experimental Study of Formula of Jiang-ya Qing-xin on Myocardial Fibrosis and Neuroendocrine in Spontaneous Hypertensive Rat

Objective ： To observe the curative effect of Formula of Jiang - ya Qing - xin on heart muscle fibrosis, AngⅡ and Ⅲ pro -collagen amino peptide （PⅢNP） in spontaneous hypertensive rat. Methods： 24 male rats of eight to ten weeks＇ age with spontaneous hypertension were divided into 4 groups randomly, which were model control group, TCM group （ group of Formula of Jiang - ya Qing - xin）, western medical group （ group of ramipril）, and integrated group （ group of Formula of Jiang - ya Qing - xin and ramipril）. There were 6 rats in each group. In addition, normal control group included 8 SD rats of eight weeks＇age. After four weeks＇ administration, AngⅡ, P ⅢNP and pathology were detected. Results ： In model control group AngⅡ and P ⅢNP were higher than those in normal control group （ P 〈 0. 01 ）, but those in TCM group, western medical group and integrated group were lower than in model control group, and significant difference showed between western medical group and integrated group and model control group （ P 〈 0. 05 ）. In normal control group cardiac muscle fibers lined up in order, andtheir size and shape were normal. In model control group cadiocytes were in overgrowth, cardiac muscle interstitial collagen fibers obviously increased, and the collagen fibers around cardiac muscle cells arranged in disorder. In TCM group myocardial interstitial was in mild hyperplasia, and cardiac muscle cells were fat lightly and moderately. In western medical group cardiac muscle fibers lined up in order, but cellular diameter transverse was swelling lightly. In integrated group cardiac interstitial collagen fibers grew downwards markedly, but intercellular collagen fibers lined up in order. Conclusion： Formula of Jiang -ya Qing- xin can inhibit heart muscle fi- brosis of spontaneous hypertensive rat, and the mechanism is to inhibit Ang Ⅱ and PⅢ NP to bring into full play.