基因疫苗预防牙周炎的实验研究

目的 将已构建的P．gingivalis kgpcd基因的真核重组表达质粒pcDNA3．1 ／kgpcd作为基因疫苗，免疫SD大鼠并观察其在预防牙周炎中的作用。方法pcDNA3．1 ／kgpcd经颌下腺靶向注射免疫SD大鼠，采用间接ELISA法检测唾液中特异性抗体的水平，并经组织化学染色观察免疫对SD大鼠牙周炎的保护情况。结果 重组质粒pcDNA3．1 ／kgpcd经颌下腺靶向注射免疫SD大鼠，可诱导机体产生高水平的唾液中抗KGPcd sIgA抗体，显著高于对照组，并且实验组牙槽骨的破坏程度显著低于对照组。结论 颌下腺靶向注射免疫可引起有效的黏膜免疫应答，KGPcd作为有效的免疫原产生的抗体对P．gingivali引起的牙周炎可起到保护作用。

Preliminary study on anti-periodontitis immunization with DNA vaccine

Objective To observe the protection against periodontal bone loss in the Sprague-Dawley (SD) rats periodontitis model, with the recombined plasmid pcDNA3.1+/kgpcd as DNA gene vaccine. Methods PcDNA3.1+/kgpcd was delivered into rats by submandibular gland-targeted injection. The anti-KGPcd sIgA in saliva was measured by indirect ELISA method.Immunohistochemistry staining was used to assess the protection in the anemal model. Results The level of specific anti-KGPcd sIgA in saliva of the experimental group was significantly higher than that of control group. HE staining showed that immunization with recombined plasmid pcDNA3.1+/kgpcd could protect or minimize tissue destruction caused by subsequent P. gingivali s challenge in the rat model. Conclusions The results indicate that pcDNA3.1+/kgpcd was a good candidate for anti-periodontitis gene vaccine and could provide protection against Porphyromonas gingivalis-caused periodontitis in rat lesion model.