抗凝药物华法林与牛血清白蛋白相互作用光谱研究

采用紫外-可见吸收光谱和荧光光谱，在生理pH条件下，研究华法林与牛血清白蛋白的相互作用。探讨华法林对于牛血清白蛋白的荧光猝灭机制。结果表明华法林对牛血清白蛋白的猝灭作用为动态猝灭过程。猝灭常数在16 ℃和37 ℃分别为6.05×10⁴ L·mol^-1和6.14×10⁴ L·mol^-1。根据Fster的偶极-偶极无辐射能量转移理论，求得牛血清白蛋白-华法林的能量转移效率E为0.37，两者间的最近距离r为3.15 nm。

Interaction between warfarin and bovine serum albumin detected by spectrometry

The interaction between warfarin and bovine serum albumin (BSA) under pseudo-physiological conditions was investigated by UV-Vis absorption spectrometry and spectrofluorimetry. The quenching mechanism of BSA by warfarin was discussed. It showed that the quenching process was a dynamic quenching, and the quenching constants were 6.05×10⁴ L·mol^-1 at 16 ℃ and 6.14×10⁴ L·mol^-1 at 37 ℃, separately. Based on the Frster theory of non-radiative energy transfer, the energy transfer efficiency and the distance of BSA to warfarin were calculated, which was 0.37 and 3.15 nm, respectively.