慢性宫内缺氧对子代兔成年期血管内皮舒张功能及血清ox-LDL、CRP的影响

目的 研究慢性宫内缺氧（CIH）对子代兔成年期血管内皮舒张功能（EVR）及血清氧化低密度脂蛋白（ox-LDL）和C反应蛋白（CRP）的影响. 方法 新西兰孕兔16只,分CIH组和非CIH组,待分娩后随机取每只母兔的雄性子代2只,母乳饲养3月后,CIH和非CIH组子代兔均再分高脂和正常饮食,即得4组：A组为CIH并高脂饮食组（CIH＆HFD）、B组为非CIH并高脂饮食组（NCIH＆HFD）、C组为CIH并正常饮食组（CIH＆ND）、D组为非CIH并正常饮食组即正常对照组（NC）,每组各8只.至6月末超声检测各组兔腹主动脉内皮舒张功能,同时检测血清ox-LDL和CRP水平. 结果 CIH可引起主动脉内皮依赖性舒张功能（EDV）降低（P＜0.05）,血清的ox-LDL和CRP水平升高（均P＜0.05）.CIH与高脂血症能协同加重上述各观察指标变化（P＜0.05或P＜0.01）.结论 CIH可能通过升高子代兔成年期血清的ox-LDL和CRP水平导致腹主动脉EDV降低,可能是动脉粥样硬化的一个重要影响因素.

The Effects of Chronic Intrauterine Hypoxia on Endothelium Vascular Relaxation and Serum ox-LDL and CRP in Adult Rabbit Offspring

Objective To investigate the effects of chronic intrauterine hypoxia on endothelium vascular relaxation and serum oxidized low-density lipoprotein（ox-LDL） and C-reactive protein（CRP） in adult rabbit offspring.Methods Sixteen New-Zealand rabbits were assigned randomly to 2 groups：chronic intrauterine hypoxia group （CIH,12%O2,n=8） and normal oxygen group （NO,21%O2,n=8）.After delivery,two male offspring rabbits per litter were selected and breast-fed for 3 months,then randomly assigned to high-fat diet or normal diet respectively.Finally,4 groups were included as follows：Chronic Intrauterine Hypoxia with High Fat Diet （CIH＆HFD,n=8）,Non-Chronic Intrauterine Hypoxia with High Fat Diet （NCIH＆HFD,n=8）,Chronic Intrauterine Hypoxia with Normal Diet （CIH＆ND,n=8） and Normal Control （NC,n=8）,which were named as group A,B,C and D respectively.At six months of age,endothelium vascular relaxation of the abdominal aorta from the offspring rabbits were evaluated by ultrasonography,and blood-lipid,serum ox LDL and CRP were examined.Results CIH resulted in an elevation of serum ox-LDL and CRP（all P〈0.05）,and an attenuation of endothelial dependent vasodilation（EDV） of abdominal aorta （P〈0.05） in adult rabbit offspring.All of these effects caused by CIH were aggravated significantly when hyperlipemia was imposed（P〈0.05 or P〈0.01）.Conclusions CIH increased the expression of ox-LDL and CRP which might impaire EDV in adult offspring,which might serve as an important factor that can induce atherosclerosis.