重症监护室耐碳青酶烯类鲍曼不动杆菌的基因分型

目的了解笔者医院重症监护室（ICU）分离的耐碳青霉烯类鲍曼不动杆菌（CRAB）的分子流行病学特点。方法收集2010年9月－2012年3月ICU分离的CRAB共34株，其中综合ICU25株，烧伤ICU9株，采用基于rep—PCR原理的DiversiLab系统进行基因分型分析。结果34株CRAB共分A、B、C和D4型，烧伤ICU中存在A和D型，以A型为主且A型仅出现在烧伤ICu；综合ICU中存在B，C和D型，以D型为主且B和C型仅出现在综合ICU。结论烧伤ICU和综合ICU中流行的CRAB基因型不同。D型CRAB同时存在两个IcU中提示ICU间可能存在交叉感染。

Genotyping of Carbapenem-resistant AcinetobacterBaumannii Strains Isolated from Intensive Care Units

Objective To investigate the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii（CRAB） strains collected from intensive care units（ICUs） in our hospital. Methods 34 CRAB strains were collected and isolated at ICUs of our hospital during the period of September 2010 to April 2012, of which 25 from general ICU, and 9 from Burn ICU. Genotyping was performed by Diversilab repetitive element PCR system. Results There were 4 types of CRAB strains, including type A, B, C and D. Type A and D CRAB strains existed in Burn ICU Type A dominated. Type B, C and D CRAB strains were found in general ICU with Type D dominant. Conclusion The epidemic strains of CRAB in Burn ICU was different from that in general ICU, and the type D CRAB strain appeared simultaneously in both the ICUs suggesting that a cross infection may prevail in the two ICUs.