人非小细胞肺癌组织p16基因缺失及突变的研究

目的　探讨人类非小细胞肺癌组织中p1 6基因缺失及突变情况及其与临床病理的关系。方法　应用控制模板DNA量的银染PCR SSCP技术检测 40例非小细胞肺癌手术切除标本中p1 6基因第 2外显子。结果　 40例中有 1 3例发生纯合缺失 ,3例发生突变 ,缺失及突变率为 40 %。其缺失及突变率与肺癌的临床分期有密切关系 (P <0 .0 5)。结论　p1 6基因的缺失及突变可能在非小细胞肺癌的发生、发展及转移中起重要作用。如

Study on the deletion and mutation of p16 gene in human non small cell lung cancer

Objective To study the relationship between the deletion and mutation of p16 gene and clinicopathologic manifestations of human non small cell lung cancer(NSCLC).Methods Silver staining PCR SSCP method of control amount of template DNA was used to detect exon 2 of p16 gene in human NSCLC tissue specimens.Results The deletion of p16 gene was identified in 13 out of 40 specimens. Of the 40 specimens, 3 showed a variant band indicative of the mutation. There was significant correlation between the frequency of the deletion and mutation (40%) and clinicopathologic stage (P<0.05).Conclusion The deletion and mutation of p16 gene may play an important role in carcinogenesis,development and metastasis of human NSCLC.