| 一种新的修饰型反义寡核苷酸抑制肿瘤细胞表达bcl-2的初步研究 |
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| 引用本文： | 秦艳芳,向军俭,刘东瀛.一种新的修饰型反义寡核苷酸抑制肿瘤细胞表达bcl-2的初步研究[J].广东医学,2002,23(6):577-579. |
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| 作者姓名： | 秦艳芳 向军俭 刘东瀛 |
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| 作者单位： | 1. 暨南大学生命科学技术学院,广州,510632
2. 武汉大学医学院,武汉,430071 |
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| 摘 要： | 目的：用新的修饰型吗呆代反义寡核苷酸（M－ODN）抑制肿瘤细胞bcl-2的表达，探讨发展新的抗肿瘤药物的可能性。方法：用吗啉代bcl-2反义寡核苷酸作用于K－562细胞，并与全硫代修饰的bcl-2反义寡核酸（S－ODN）为对比，MTT法观察细胞生长抑制率，流式细胞术观察细胞凋亡率。结果：吗啉代修饰型的bcl-2反义寡核苷酸在作用效率、持久性及低细胞毒性等方面均优于硫代修饰。结论：反义吗啉代寡核有望成为新的肿瘤基因治疗手段。
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| 关 键 词： | 修饰型反义寡核苷酸 肿瘤细胞 bcl－2 基因表达 |
| 修稿时间： | 2001年11月15 |
Inhabition of bcl-2 expression by antisense morpholino olgodexynucleotide |
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| Qin Yanfang,Xiang Junjian,Liu Dongying. Life Science and Technology College of Jinan University,Guangzhou.Inhabition of bcl-2 expression by antisense morpholino olgodexynucleotide[J].Guangdong Medical Journal,2002,23(6):577-579. |
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| Authors: | Qin Yanfang Xiang Junjian Liu Dongying Life Science and Technology College of Jinan University Guangzhou |
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| Institution: | Qin Yanfang,Xiang Junjian,Liu Dongying. Life Science and Technology College of Jinan University,Guangzhou 510632 |
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| Abstract: | Objective To investigate the anti-tumor effects of bcl-2 antisense morpholino oligodexynucleotide(M-ODN). Methods MTT spetrophotometry and flow cytometry were used to examine the effect of M-ODN on survival of K-562 cells and results were compared with that of phosphorothioate oligodexynucleotide(S-ODN). Results M-ODN offered many advantages over S-ODN in inhibitive efficacy,persistence and safety profile. Conclusion bcl-2 antisense M-ODN may become a new anti-tumor approach. |
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| Keywords: | Antisense inhibition Olgodexynucleotide Tumor bcl-2 |
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