重组人转化生长因子β3对成纤维细胞作用的观察

目的　观察重组人转化生长因子 β3(recombinehumantransforminggrowthfactorβ3,rhTGFβ3)对成纤维细胞的作用 ,探讨其可能机制。　 方法　取体外培养的人正常皮肤成纤维细胞(normalskinfibroblast,NSFb)和增生性瘢痕成纤维细胞 (hypertrophicscarfibroblast,HSFb) ,经不同浓度的rhTGFβ3处理 ;另设不含rhTGFβ3的DMEM培养液相应细胞组作为对照。通过放射免疫和Northernblot杂交法 ,观察NSFb和HSFb中Ⅰ、Ⅲ型胶原蛋白及转录水平mRNA表达的变化。　结果　 (1)NSFb和HSFb中Ⅰ、Ⅲ型前胶原的表达有明显不同 ;(2 )与对照组相比 ,经rhTGFβ3处理的各实验组Ⅰ、Ⅲ型前胶原合成明显增加 (P <0 .0 0 1) ,Ⅰ Ⅲ型前胶原的比例变小 ;(3)rhTGFβ3对NSFb和HSFb生物学作用的影响呈明显的量效关系。在相同剂量作用下 ,HSFb的PCⅠ、PCⅢ含量高于NSFb。　结论　rhTGFβ3能有效提高成纤维细胞中Ⅰ、Ⅲ型胶原的合成和Ⅰ、Ⅲ型构成比中Ⅲ型胶原的相对含量 ,可能对加速创面愈合及减少瘢痕形成具有重要的作用

An observation of the effects of recombinant human transforming growth factor β3 on fibroblast

OBJECTIVE: To investigate the role of recombinant human transforming growth factor beta3 (rhTGFbeta3) on fibroblast and its possible mechanism. METHODS: Normal skin fibroblast (NSFb) and hypertrophic scar fibroblast (HSFb) were cultured in vitro, and were processed by different concentrations of rhTGFbeta3. NSFb and HSFb in DMEM solution without rhTGFbeta3 were employed as control. The changes in the protein and mRNA expression of type I and III collagen in NSFb and HSFb were observed. RESULTS: (1) The expression of type I and III procollagen in NSFb was evidently different from that of HSFb (2) The synthesis of type I and III procollagen in all test groups was increased obviously after rhTGFbeta3 process (P < 0.001) while the ratio of type I to III procollagen was decreased when compared with that in control group. (3) The effects of rhTGFbeta3 on the biological behavior exhibited an obvious dose- effects relationship. The contents of type I to III procollagen in HSFb were higher than those in NSFb when the dose of rhTGFbeta3 was same. CONCLUSION: rhTGFbeta3 could effectively promote the synthesis of type I and III procollagen, especially type III procollagen in fibroblasts. This might be beneficial to the accelerate of wound healing and to inhibit or prevent scar formation.