A rapid method for purification of human granulocytes using percoll. A comparison with dextran sedimentation |
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| Authors: | R Hjorth A K Jonsson P Vretblad |
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| Affiliation: | Cell Biology Group, Pharmacia Fine Chemicals AB, Box 175, S-751 04 Uppsala, Sweden |
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| Abstract: | A simple one-step method for purification of human granulocytes, based on the use of a discontinuous gradient of Percoll, is described. The cell yield is 55% and the purity of the granulocyte fraction is 97%. The cells' ability to exclude trypan blue and phagocytic function are not altered during the purification process. We conclude that centrifugation in Percoll is superior to conventional dextran sedimentation; it is also less time-consuming. |
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