VP-16诱导前列腺癌细胞PC-3凋亡的研究

为研究ＶＰ－１６对雄激素非依赖性前列腺癌细胞ＰＣ－３凋亡的诱导作用，应用ＨＥ染色观察细胞的形态学改变，以ＤＮＡ凝胶电泳和流式细胞术（ＦＣＭ）分析细胞凋亡和细胞周期分布，同时应用３Ｈ－ＴｄＲ掺入法研究ＶＰ－１６对ＰＣ－３细胞ＤＮＡ合成的影响。结果表明，（１）凋亡的ＰＣ－３细胞呈明显的形态学改变；（２）ＤＮＡ断裂呈梯形变化；（３）ＰＣ－３细胞凋亡率随ＶＰ－１６作用浓度增加和时间延长逐渐增高；（４）ＶＰ－１６能够明显抑制ＰＣ－３细胞ＤＮＡ的合成。提示ＶＰ－１６可诱导前列腺肿瘤细胞凋亡。

VP16 INDUCED APOPTOSIS IN PC3 CELLS

To study VP16 induce apoptosis in PC3 cells, whose growth is androgen independent. The change of cell morphology was observed by HE staining, DNA gel electrophoresis and flow cytometry(FCM) were applied to investigate the apoptosis in PC3 cells and distribution of cell cycle, DNA synthesis was detected by 3KG*2]HTdR penetration. HE staining showed typical apoptotic morphological changes in PC3 cells; DNA cleavage in PC3 cell presented ladder lanes, the percentage of apoptotic cells was increased with the increasing of concentration and exposure time of VP16; DNA synthesis in PC3 cells was strongly inhibited by VP16. These findings suggest that VP16 can induce apoptosis in prostatic cancer.