Oxygen generating scaffolds for enhancing engineered tissue survival |
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| Authors: | Se Heang Oh Catherine L Ward Anthony Atala James J Yoo Benjamin S Harrison |
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| Institution: | 1. Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran;2. National Cell Bank, Pasteur Institute of Iran, Tehran, Iran;3. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;4. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran;1. Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital Shanghai, China;2. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, China;3. Shanghai Eastern Urological Reconstruction and Repair Institute, Shanghai, China;1. Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA;2. Diabetes Research Institute, University of Miami, Miami, FL, USA;3. Department of Biomedical Engineering, University of Miami, Miami, FL, USA;4. Department of Biochemistry and Molecular Biology, University of Miami, Miami, FL, USA;1. Biomaterial Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, Iran;2. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia;3. Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;4. Bioengineering Research Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), Tehran, Iran;1. Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA;2. Department of Emergency Medicine, University of Michigan, Ann Arbor, MI 48109, USA;3. Michigan Center for Integrative Research in Critical Care, University of Michigan, Ann Arbor, MI 48109, USA;1. Department of Chemical and Biomolecular Engineering, University of Akron, OH 44325, USA;2. Department of Chemistry, University of Akron, OH 44325, USA;3. Summa Akron City Hospital, Akron, OH 44304, USA;4. Akron General Medical Center, Akron, OH 44307, USA |
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| Abstract: | One of the continued challenges in engineering clinically applicable tissues is the establishment of vascularization upon implantation in vivo. Although the effectiveness of an enhanced angiogenic response using various growth factors has been demonstrated in many tissue systems, the rate of angiogenesis could not be accelerated. In this study we investigated whether incorporating oxygen generating biomaterials into tissue engineered constructs would provide a sustained oxygen release over an extended period of time. We examined whether oxygen generating biomaterials are able to maintain cell viability while also maintaining structural integrity of a 3-D construct. Calcium peroxide-based oxygen generating particles were incorporated into 3-D scaffolds of Poly(d,l-lactide–co–glycolide) (PLGA). The scaffolds were designed to generate oxygen over the course of 10 days and simultaneously maintain sufficient mechanical integrity. Scaffolds containing oxygen generating materials maintained elevated levels of oxygen when incubated under hypoxic conditions. Further, these biomaterials were able to extend cell viability growth under hypoxic conditions. These findings indicate that the use of oxygen generating biomaterials may allow for increased cell survivability while neovascularization is being established after implantation. Such scaffolds may play an important role in tissue engineering where currently oxygen diffusion limits the engineering of large tissue implants. |
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