Regulation of cellular morphology using temperature-responsive hydrogel for integrin-mediated mechanical force stimulation |
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| Authors: | Kazumasa Yamaki Ichiro Harada Mitsuaki Goto Chong-Su Cho Toshihiro Akaike |
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| Institution: | 1. Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-57 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan;2. Celagix Res. Ltd., Japan;3. School of Agricultural Biotechnology, Seoul National University, South Korea;1. Institute of Medical Device and Imaging, National Taiwan University, Taipei, Taiwan;2. Graduate Institute of Electronics Engineering, National Taiwan University, Taipei, Taiwan;1. Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA;2. Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland;3. Department of Biomedical Engineering, National University of Ireland, Galway, Ireland;4. Department of Bioengineering, Stanford University, Stanford, CA, USA;5. Trinity Centre for Bioengineering, Trinity College Dublin (TCD), Dublin, Ireland;6. Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, Dublin, Ireland;1. Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 6997801, Israel;2. Faculty of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa 3200003, Israel;1. Group of Epigenetic Reprogramming, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China |
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| Abstract: | A new culture substrate was developed for cells to be equibiaxially stretched using fibronectin (Fn)-immobilized temperature-responsive hydrogel. The cells cultured on the gel substrate were equibiaxially stretched with swelling of the gel, which was accompanied by slight changes of temperature. During gel swelling, changes of cell shape were clearly observed by optical microscopy because of high transparency of the gel. ERK was highly and transiently activated by mechanical stimulation whereas focal adhesion kinase (FAK) was not, indicating that mechanical signals were transduced into biochemical signals in cells. We found that cells formed filopodia-like structures in response to mechanical cues, suggesting that mechanical forces facilitated actin polymerization at the peripheral region. In the cytoplasm, paxillin-containing fibrous structures were formed along actin fibers. These results indicate that we can perform both analysis of intracellular signal transduction and observation of cell shapes at high magnification in our method. |
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