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The effect of RGD density on osteoblast and endothelial cell behavior on RGD-grafted polyethylene terephthalate surfaces
Authors:Celine Chollet  Christel Chanseau  Murielle Remy  Alain Guignandon  Reine Bareille  Christine Labrugère  Laurence Bordenave  Marie-C Durrieu
Institution:1. INSERM, U577, Univ. Victor Segalen Bordeaux 2, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France;2. Centre de Caractérisation des Matériaux Avancés, ICMCB-CNRS, Université Bordeaux 1, Avenue du Docteur Schweitzer, 33608 Pessac, France;3. INSERM, U890, St-Etienne, F-42023 France; Univ. Jean Monnet, France, F-42023 France, 15 rue Ambroise PARE, 42023 St-Etienne Cedex2, France;4. INSERM, U802 Bordeaux, CIC-IT and CHU Bordeaux, Hôpital Xavier Arnozan, Pessac F-33604, France;1. Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia;2. Department of Functional Materials in Medicine and Dentistry, Universitätsklinikum Würzburg, Pleicherwall 2, 97070 Würzburg, Germany;1. Laboratoire d’Ingénierie de Surface, Centre de Recherche sur les Matériaux Avancés, Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, 1065 Avenue de la médecine, Québec, G1V 0A6, Canada;2. Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d’Assise, 10 rue de l’Espinay, Québec, G1L 3L5, Canada;3. Institute of Chemistry & Biology of Membranes & Nanoobjects (CNRS, UMR5248 CBMN), Université de Bordeaux, Bordeaux INP, France;4. Institute of Biomaterials and Biomedical Engineering, Department of Materials Science and Engineering, and Faculty of Dentistry, University of Toronto, Toronto, ON M5S 3G9, Canada;1. Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany;2. Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332–0363, USA;1. “Lvivska Polytechnika” National University, S. Bandery 12, 79013 Lviv, Ukraine;2. Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland;3. Lviv Academy of Commerce, Samtshuk 9, and Lviv Institute for Physical Optics, Dragomanov 19, 79011 Lviv, Ukraine;4. AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, al. A. Mickiewicza 30, 30-059 Kraków, Poland;1. Department of Chemical Engineering, National Taiwan University, No.1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan;2. Department of Chemical Engineering, National Taiwan University of Science and Technology, No.43, Keelung Rd., Sec. 4, Taipei 106, Taiwan
Abstract:Hybrid materials combining polyethylene terephthalate and different types of cells (endothelial and osteoblastic cells) have been developed thanks to the covalent grafting of different densities of RGD containing peptides onto the polymer surface. Biomimetic modifications were performed by means of a three-step reaction procedure: creation of COOH functions, coupling agent grafting and the immobilization of the RGDC peptides. High resolution μ-imager was used to evaluate RGD densities (varying between 0.6 and 2.4 pmol/mm2) and has exhibited the stability of the surface grafted peptides when treated in harsh conditions. The efficiency of this route for biomimetic modification of a PET surface was demonstrated by measuring the adhesion of MC3T3 and HSVEC cells and by focal adhesion observation. Results obtained prove that a minimal RGDC density of 1 pmol/mm2 is required to improve MC3T3 and HSVEC cells responses. Indeed, cells seeded onto a RGDC-modified PET with a density higher than 1 pmol/mm2 were able to establish focal adhesion as visualized by fluorescence microscope compared to cells immobilized onto unmodified PET and RGDC-modified PET with densities lower than 1 pmol/mm2. Moreover, the number of focal contacts was enhanced by the increase of RGDC peptide densities grafted onto the material surface. With this study we proved that the density of peptides immobilized on the surface is a very important parameter influencing osteoblast or endothelial cell adhesion and focal contact formation.
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