Mesenchymal stem cell differentiation to neuronal cells on electrospun nanofibrous substrates for nerve tissue engineering |
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| Authors: | Molamma P. Prabhakaran Jayarama Reddy Venugopal Seeram Ramakrishna |
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| Affiliation: | 1. College of Science, University of Shanghai for Science & Technology, Shanghai, PR China;2. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, PR China;3. CQM-Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, Funchal, Portugal;4. State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, People''s Republic of China;1. Instituto de Telecomunicações, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;2. Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;3. iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;1. Faculty of Biomaterial and Tissue Engineering, Department of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, P.O. Box: 15875/4413, Tehran 159163/4311, Iran;2. Department of Material science & Engineering, Amirkabir University of Technology, Tehran, Iran;1. Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran;2. Department of Mechanical and Aerospace Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran;3. Oral and Maxillofacial Surgery Department, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4. School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran;5. Department of Medical Nanotechnology, School of Advanced Technology in Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran;6. International Medicine Department, Aja University of Medical Sciences, Tehran, Iran;7. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran;8. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran |
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| Abstract: | Bone marrow Mesenchymal stem cells capable of differentiating into neuronal cells on engineered nanofibrous scaffolds have great potential for bionanomaterial–cell transplantation therapy of neurodegenerative diseases and injuries of the nervous system. MSCs have been the highlight of many tissue engineering studies mainly because of their multipotential properties. We investigated the potential of human bone marrow derived Mesenchymal stem cells (MSCs) for neuronal differentiation in vitro on poly(l-lactic acid)-co-poly-(3-caprolactone)/Collagen (PLCL/Coll) nanofibrous scaffolds. PLCL and PLCL/Coll nanofibrous scaffolds were fabricated by electrospinning process and their chemical and mechanical characterizations were carried out using SEM, contact angle, FTIR, and tensile instrument. The differentiation of MSCs was carried out using neuronal inducing factors including β-mercaptoethanol, epidermal growth factor, nerve growth factor and brain derived growth factor in DMEM/F12 media. The proliferations of MSCs evaluated by MTS assay showed that the cells grown on PLCL/Coll nanofibrous scaffolds were comparatively higher (80%) than those on PLCL. Scanning electron microscopy results showed that MSCs differentiated on PLCL/Coll nanofibrous scaffolds showed neuronal morphology, with multipolar elongations and expressed neurofilament and nestin protein by immuno-fluorescent microscopy. Our studies on the differentiation of MSCs to neuronal cells on nanofibrous scaffolds suggest their potential application towards nerve regeneration. |
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