Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering |
| |
| Authors: | ?árka Kubinová Daniel Horák Eva Syková |
| |
| Institution: | 1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague 4, Czech Republic;2. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic;3. Center for Cell Therapy and Tissue Repair, Charles University, V Úvalu 84, 150 06 Prague 5, Czech Republic;1. Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt;2. Nuclear Materials Authority, El Maadi, Cairo, Egypt;1. Department of Biomedical Science, Daegu University, Gyeongsan 712-714, South Korea;2. Department of Physics, Daegu University, Gyeongsan 712-714, South Korea;3. Center for Bio-Nanomaterials, Daegu University, Gyeongsan 712-714, South Korea;4. Department of Biotechnology, Hannam University, Daejeon 305-811, South Korea;1. Department of Pharmaceutical Technology, Faculty of Pharmacy, “Gr. T. Popa” University of Medicine and Pharmacy, 16 Universit??ii Street, 700115 Ia?i, Romania;2. Laboratory of Polyaddition and Photochemistry, “Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Vod? 41 A, 700487 Ia?i, Romania;3. Department of Organic, Biochemical and Food Engineering, Faculty of Chemical Engineering and Environmental Protection, “Gheorghe Asachi” Technical University of Ia?i, Mangeron Avenue 73, 700050 Ia?i, Romania;4. Department of Natural and Synthetic Polymers, Faculty of Chemical Engineering and Environmental Protection, “Gheorghe Asachi” Technical University of Ia?i, Mangeron Avenue 73, 700050 Ia?i, Romania;5. Center of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Vod? 41 A, 700487 Ia?i, Romania;6. Laboratory of Physical Chemistry of Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Vod? 41 A, 700487 Ia?i, Romania;7. Polymer Materials Physics, “Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Vod? 41 A, 700487 Ia?i, Romania;8. Microbiology Department, Faculty of Medicine, “Gr. T. Popa” University of Medicine and Pharmacy, 16 Universit??ii Street, 700115 Ia?i, Romania;1. Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indianapolis University-Purdue University Indianapolis, Indianapolis, IN 46202, USA;2. Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea;3. Department of Medicine and Biochemistry and Molecular Biology, Indiana University School of Medicine, The Melvin and Bren Simon Cancer Center and the Center for Pancreatic Cancer Research, Indianapolis, IN 46202, USA;1. College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;2. The State Key Laboratory Breeding Base of Basic Science of Stomatology, Hubei Province & Key Laboratory of Oral Biomedicine (Wuhan University), Wuhan 430079, China |
| |
| Abstract: | Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with cholesterol and laminin have been developed to design scaffolds that promote cell–surface interaction. Cholesterol-modified superporous PHEMA scaffolds have been prepared by the bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA), cholesterol methacrylate (CHLMA) and the cross-linking agent ethylene dimethacrylate (EDMA) in the presence of ammonium oxalate crystals to introduce interconnected superpores in the matrix. With the aim of immobilizing laminin (LN), carboxyl groups were also introduced to the scaffold by the copolymerization of the above monomers with 2-(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA). Subsequently, the MCMEMA moiety in the resulting hydrogel was hydrolyzed to 2-(methacryloyloxy)ethoxy]acetic acid (MOEAA), and laminin was immobilized via carbodiimide and N-hydroxysulfosuccinimide chemistry. The attachment, viability and morphology of mesenchymal stem cells (MSCs) were evaluated on both nonporous and superporous laminin-modified as well as laminin-unmodified PHEMA and poly(2-hydroxyethyl methacrylate-co-cholesterol methacrylate) P(HEMA–CHLMA) hydrogels. Neat PHEMA and laminin-modified PHEMA (LN–PHEMA) scaffolds facilitated MSC attachment, but did not support cell spreading and proliferation; the viability of the attached cells decreased with time of cultivation. In contrast, MSCs spread and proliferated on P(HEMA–CHLMA) and LN-P(HEMA–CHLMA) hydrogels. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
