The influence of degradation characteristics of hyaluronic acid hydrogels on in vitro neocartilage formation by mesenchymal stem cells |
| |
| Authors: | Cindy Chung Michael Beecham Robert L. Mauck Jason A. Burdick |
| |
| Affiliation: | 1. Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321, USA;2. McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA;1. Department of Bioengineering, Rice University, Houston, TX 77251-1892, USA;2. Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan;1. Division of Advanced Prosthodontics, University of California, Los Angeles, CA 90095, USA;2. Department of Bioengineering, University of California, Los Angeles, CA 90095, USA;3. Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA;4. Division of Diagnostic and Surgical Sciences, University of California, Los Angeles, CA 90095, USA;5. Department of Bio and Chemical Engineering, Hongik University, Sejong 339-701, Republic of Korea;6. Department of Orthopaedic Surgery, University of California, Los Angeles, CA 90095, USA;1. Bioengineering Department, University of California Los Angeles, Los Angeles, CA 90095, USA;2. Chemical and Biomolecular Engineering Department, University of California Los Angeles, Los Angeles, CA 90095, USA;1. Department of Orthopaedics, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands;2. Center for Nanohealth, Swansea University Medical School, Wales, United Kingdom;3. Department of Plastic and Reconstructive Surgery, University Medical Center Utrecht, Utrecht, The Netherlands;4. Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands;1. Department of Bioengineering, University of California, Berkeley, CA 94720, USA;2. Department of Material Science and Engineering, University of California, Berkeley, CA 94720, USA;3. Department of Nutritional Science and Toxicology, University of California, Berkeley, CA 94720, USA;4. Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway, Ireland;5. Department of Medicine, University of California, San Francisco, CA 94143, USA;6. Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA;7. Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA |
| |
| Abstract: | The potential of mesenchymal stem cells (MSCs) as a viable cell source for cartilage repair hinges on the development of engineered scaffolds that support adequate cartilage tissue formation. Evolving networks (hydrogels with mesh sizes that change over time due to crosslink degradation) may provide the control needed to enhance overall tissue formation when compared to static scaffolds. In this study, MSCs were photoencapsulated in combinations of hydrolytically and enzymatically degradable hyaluronic acid (HA) hydrogels to investigate the tunability of these hydrogels and the influence of network evolution on neocartilage formation. In MSC-laden HA hydrogels, compressive mechanical properties increased when degradation complemented extracellular matrix deposition and decreased when degradation was too rapid. In addition, dynamic hydrogels that started at a higher wt% and decreased to a lower wt% were not equivalent to static hydrogels that started at the higher or lower wt%. Specifically, evolving 2 wt% hydrogels (2 wt% degrading to 1 wt%) expressed up-regulation of type II collagen and aggrecan, and exhibited increased glycosaminoglycan content over non-evolving 2 and 1 wt% hydrogels. Likewise, mechanical properties and size maintenance were superior in the dynamic system compared to the static 2 wt% and 1 wt% hydrogels, respectively. Thus, hydrogels with dynamic properties may improve engineered tissues and help translate tissue engineering technology to clinical application. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
