The effect of mesoporous bioactive glass on the physiochemical,biological and drug-release properties of poly(dl-lactide-co-glycolide) films |
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| Authors: | Chengtie Wu Yogambha Ramaswamy Yufang Zhu Rongkun Zheng Richard Appleyard Andrew Howard Hala Zreiqat |
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| Institution: | 1. Biomaterials and Tissue Engineering Research Unit, School of AMME, The University of Sydney, Sydney 2006, Australia;2. Bosch Institute, The University of Sydney, Sydney 2006, Australia;3. ICYS-Sengen, National Institute for Materials Science, 1-2-1 Segen, Tsukuba, Ibaraki 305-0047, Japan;4. EMU, The University of Sydney, Sydney, 2006 NSW, Australia;5. Murray Maxwell Biomechanics Laboratory, Kolling Institute (University of Sydney), Royal North Shore Hospital, Sydney, 2065 NSW, Australia;1. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China;2. Institute of Health & Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland 4059, Australia;1. Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China;2. Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, Erlangen 91058, Germany;3. Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Martenstrasse 7, Erlangen 91058, Germany;4. Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen 361005, PR China;5. Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, The Key Laboratory of Biomedical Material of Tianjin, Tianjing 300192, PR China;6. Research Center of Biomedical Engineering, Department of Biomaterials, Xiamen University, The Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering Technology of Xiamen City, Xiamen, Fujian 361005, PR China;1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea;2. Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea;3. Glass Research Department, National Research Center, Cairo 12622, Egypt;4. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea;1. Hubei Key Laboratory of Processing and Application of Catalytic Materials, College of Chemical Engineering, Huanggang Normal University, No. 146, Xingang 2 Road, Huanggang City, Hubei Province, 438000, China;2. School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai, 200093, China;3. Shanghai Innovation Institute for Materials, Shanghai, 200444, China;1. Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain;2. CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Madrid, Spain;3. Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 28040 Madrid, Spain;1. Universidad de Buenos Aires, Faculty of Pharmacy and Biochemistry, Pharmaceutical Technology Department, Buenos Aires, Argentina;2. CONICET (National Research Council), Argentina;3. Institute of Biomaterials, University of Erlangen-Nuremberg, 91058 Erlangen, Germany |
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| Abstract: | Poly(lactide-co-glycolide) (PLGA) has been widely used for bone tissue regeneration. However, it lacks hydrophilicity, bioactivity and sufficient mechanical strength and its acidic degradation by-products can lead to pH decrease in the vicinity of the implants. Mesoporous bioactive glass (MBG) with highly ordered structure (pore size 2–50 nm) possesses higher bioactivity than non-mesoporous bioactive glass (BG). The aim of this study is to investigate the effect of MBG on the mechanical strength, in vitro degradation, bioactivity, cellular response and drug release of PLGA films and optimize their physicochemical, biological and drug-delivery properties for bone tissue engineering application. The surface and inner microstructure, mechanical strength and surface hydrophilicity of MBG/PLGA and BG/PLGA films were tested. Results indicated that MBG or BG was uniformly dispersed in the PLGA films. The incorporation of MBG into PLGA films significantly improved their tensile strength, modulus and surface hydrophilicity. MBG/PLGA resulted in an enhanced mechanical strength, in vitro degradation (water absorbance, weight loss and ions release), apatite-formation ability and pH stability in simulated body fluids (SBF), compared to BG/PLGA. MBG/PLGA and BG/PLGA films enhanced human osteoblastic-like cells (HOBs) attachment, spreading and proliferation compared to PLGA. HOBs differentiation was significantly upregulated when cells were cultured on 30 MBG/PLGA for 14 days, compared to 30 BG/PLGA. MBG/PLGA enhanced the accumulative release of dexamethazone (DEX) at early stages (0–200 h) compared to BG/PLGA, however, after 200 h, DEX-release rates for MBG/PLGA was slower than that of BG/PLGA. The contents of MBG in PLGA films can control the amount of DEX released. Taken together, MBG/PLGA films possessed excellent physicochemical, biological and drug-release properties, indicating their potential application for bone tissue engineering by designing 3D scaffolds according to their corresponding compositions. |
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