肾移植病房耐甲氧西林金黄色葡萄球菌随机扩增多态DNA分型研究

目的 研究医院肾移植病房耐甲氧西林金黄色葡萄球菌(MRSA)的分子流行病学特征,以确定病房内是否存在MRSA流行.方法 用头孢西丁纸片扩散法检测MRSA,用双重PCR方法检测金黄色葡萄球菌的femA,mecA基因,采用随机扩增多态DNA技术(RAPD),对肾移植病房一段时间内临床患者感染部位及环境和医务人员分离的MRSA作同源性分析.结果 16例患者中有5例感染MRSA；从57份医护人员鼻腔、手部、环境分离出1株MRSA；5株患者MRSA的RAPD分型具有较高的同源性；医护人员分离的MRSA与患者分型结果无同源性.结论我们医院肾移植病房存在严重的MRSA局部流行,应及时检测MRSA,防止医院感染菌株的播散.

Genotyping of meticillin-resistant Stophylococcs aureus by randomly amplified polymorphic DNA in kidney transplant department

OBJECTIVE To investigate the characteristics of molecular epidemiology of meticillin-resistant Staphylococcus aureus(MRSA) strains in kidney transplant department so as to verify the prevalence of MRSA strains.METHODS MRSA isolates were detected by cefoxitin disk diffusion,and double-PCR was adopted to detect mecA and femA genes in Staphylococcus aureus,then randomly amplified polymorphic DNA(RAPD) was applied to analyze the homology of MRSA strains which were isolated from the infection sites of the hospitalized patients,environment,as well as doctors and nurses in the kidney transplant department.RESULTS There were 5 of 16 patients who were infected with MRSA;one MRSA strain was isolated from 57 samples including the air,the nares and hands of the healthcare workers;the genotyping of RAPD of 5 strains of MRSA was highly homologous;there was no homology of genotyping of the MRSA strains isolated from the healthcare workers and the patients.CONCLUSION There exists the wide spread of MRSA isolates in the kidney transplant department of our hospital,it is necessary to detect MRSA in time so as to prevent the spread of the bacteria causing nosocomial infections.