泪腺多形性腺瘤ras癌基因产物表达与DNA含量的关系

目的:泪腺多形性腺瘤是常见的泪腺肿瘤,约占上皮性肿瘤的50％。手术后常有复发,恶性变也不少见。以往的研究表明,肿瘤的发生发展与癌基因产物的异常表达有关。本研究的目的是观察泪腺多形性腺瘤是否存在有P~(21ras)的异常表达。方法:研究采用免疫组化ABC法测定5例正常泪腺组织及32例多形性腺瘤P~(21ras)的表达,抗体选用单克隆抗体F—132—62。肿瘤细胞核DNA含量测定采用计算机图像分析技术。结果:正常泪腺组织均染色阴性,12例肿瘤染色阳性。14例肿瘤细胞核DNA含量增加,其倍体分布类型呈双峰及多峰型。P~(21ras)表达与肿瘤细胞DNA含量倍体分布类型明显相关,在P~(21ras)染色阳性的肿瘤,DNA含量倍体分布类型主要为双峰及多峰型,经精确概率x~2检验,差异有显著性,P<0.05。结论:本研究的结果提示,泪腺多形性腺瘤有P~(21ras)的增强表达,P~(21ras)的异常表达与肿瘤的发展有关。眼科学报 1996;12;54—57。

Relationship between Expression of P~(21ras) and Cellular DNA in Pleomorphic Adenoma of Lacrimal Gland

Background: The pleomorphic adenoma is the most frequent tumor of the human lacrimal gland comprising about 50% of the epithelial tumors of this organ. Although being benign, local recurrences can occur when the first removal was imcomplete and malignant transformation is also not in frequent. It is well known that many sorts of cellular oncogene products are involved in the initiation, promotion and progression of the human neoplasm. Our purpose was to know whether there is abnormal expression of P21ras in pleomorphic adenoma. Methods:We have undertaken a study of the expression of P21rasin 5 normal tissues and 32 pleomorphic adenoma, of lacrimal gland by immunohistochemical means using the monoclone antibody F-132-62 and the nuclear DNA content in the tumor was assayed by image analysis technique.Results:Normal tissues, of lacrimal gland were negative, 12 tumors were stained positively with the antibody. The DNA content of 14 cases of tumor was increased, their DNA ploidy distribution pattern showed two or several peaks. Good correlation has been found between the expression of P21rasand DNA ploidy distribution pattern, the DNA ploidy distribution pattern of tumor which expressed P21ras showed mainly two or several peaks. P<0. 05. Conclusions: The result of our studies may suggest that there are increased expression of P21ras in pleomorphic adenoma and the expression of p21ras is related to the promotion and progression of pleomorphic adenoma of lacrimal gland. Eye Science 1996; 12: 54- 57.