合成油的营养评价——(二)合成油饲料对实验性高胆固醇血症大鼠体重、血脂和肝脂成分的影响

在88g基础饲料中加入12g不同种类的油脂(合成油、猪油和豆油)和1％胆固醇喂养大鼠5周以诱导动物产生高脂血症,并以不加入胆固醇的豆油饲料作为正常对照。比较各组动物在实验期内的生长、血清脂质、肝脏脂质和某些内脏器官重量的改变。结果如下: (1)大鼠摄食含不同油脂的致高胆固醇血症饲料5周后,各组雄鼠的体重增加无明显的不同,雌鼠的体重增加则有显著的差别,即合成油组动物的增重显著较猪油组和对照组的为低。 (2)在各种油脂饲料中加入胆固醇都可使动物的肝重增大。猪油组和豆油组动物的肝脏有明显的脂肪肝病变。肝脂含量增加,肝胆固醇和甘油三酯大量积存(分别约为对照组的9-10倍和2-3倍)。而合成油组动物的肝脏外观表现正常,肝脂含量、肝胆固醇和甘油三酯水平则接近对照组的正常水平。 (3)在各种油脂饲料中加入胆固醇喂养大鼠,对雄鼠血清胆固醇水平的影响不明显,但引起雌鼠血清胆固醇的改变和对照组比较,猪油饲料使动物的血清胆固醇含量明显增高,豆 油和合成油饲料则可维持动物的血胆固醇含量于正常水平。血清甘油三酯水平则以合成油饲料组动物的为最高。 (4)在各种饲料中加入胆固醇除增大肝脏重量外,也使雌鼠的主动脉重量增高,但饲料油脂种类对其重量的影响不明显。 上述结果表明,当饲料中含有高水?

NUTRITIONAL EVALUATION OF SYNTHETIC OIL——Ⅱ. Effect of Synthetic Oil on Body Weight Gain,Blood Cholesterol,Liver Weight,Liver Fat and Liver Cholesterol Level During the Inducing Period of Hypercholesterolemia

The effects of synthetic oil used as dietary fat source on the body weight, organ weight, liver and serum lipids of rats during the hypercho-lesterolemic inducing period were investigated, and comparison was made with lard and soybean oil diets.Weanling albino rats were fed first with stock diet for 3 weeks. Bloods were collected from tails, and serum cholesterol contents were determined. Based upon the body weight and serum cholesterol level, animals were then divided into 5 groups. Four groups were fed respectively with synthetic oil, synthetic oil plus linoleic acid, lard and soybean oil diets, all were supplemented with 1% cholesterol. The, fifth group served as control, was fed with soybean oil diet without cholesterol. Body weights and serum lipids (cholesterol and triglyceride) were measured at intervals during the experiment. Organ weights and liver lipids (total fat, cholesterol and triglyceride) were determined at the end of the experiment.The results showed that there was marked sex difference between male and female rats, that is, synthetic oil diet caused significant lower body weights and lower serum cholesterol levels to female but not to male rats.Cholesterol inducing diets apparently increased the liver weights of all animals, yet fat accumulation only occured in those livers of rats fed with lard and soybean oil diets. The liver fat contents of animals of both synthetic oil and synthetic oil plus linoleic acid groups were at normal level.This experiment reveals that when dietary cholesterol intake is high, the use of synthetic oil as a dietary fat source for maintaining a normal lipid metabolic condition in the body seems beneficial.