慢病毒PARG-shRNA转染降低大肠癌lovo细胞基质黏附、运动和侵袭能力

目的探讨聚腺苷二磷酸核糖水解酶(PARG)基因沉默对人大肠癌lovo细胞基质黏附、运动和侵袭能力的影响。方法慢病毒PARG-shRNA转染人大肠癌lovo细胞并筛选出稳定沉默PARG基因的lovo细胞株;Western blot法检测PARG、PARP和NF-κB的表达。用细胞基质黏附、运动和侵袭实验观察lovo细胞基质黏附、运动和侵袭能力。结果获得了稳定的PARG基因沉默lovo细胞株,实验组PARP和NF-κB的表达显著降低(P<0.05)。PARG沉默后lovo细胞黏附、运动和侵袭能力降低。和未转染组相比,其黏附、运动和侵袭抑制率分别为25.22%、38.71%和35.29%。结论PARG基因沉默可以降低lovo细胞基质黏附、运动和侵袭能力,此可能与PARG沉默后下调PARP,并下调NF-κB的活性有关。提示PARG在肿瘤侵袭和转移中可能发挥重要作用。

Lentivirus PARG-shRNA transfection decreases matrix adhesion,migration and invasion potencies of colon carcinoma lovo cells

Objective To explore the effects of poly(ADP-ribose) glycohydrolase (PARG) gene silencing on colon carcinoma lovo cells matrix adhesion,migration and invasion.Methods Lentivirus PARG-shRNA was transfected into colon carcinoma lovo cells and the lovo cell strain with PARG gene silencing perpetually was selected.Western blot was used to test the expression of PARG,poly(ADP-ribose) polymerase(PARP) and NF-κB.The lovo cell matrix adhesion,migration and invasion potencies were observed by cell matrix adhesion,migration and invasion assay.Results The expression of PARP and NF-κB in experiment groups was weaker than that in the control groups (P＜0.05).PARG gene silencing of lovo cells decreased the cell matrix adhesion,migration and invasion potencies.The inhibitory rates of lovo cell matrix adhesion,migration and invasion were 25.22%,38.71%,35.29% respectively.Conclusion PARG gene silencing of lovo cells could inhibite cell matrix adhesion,migration and invasion potencies,which may relate to that PARG gene silencing and decreasing the activity of PARP and NF-κB.PARG probably plays an important role in the infiltration and metastasis of tumor.