咖啡豆提取物对肥胖大鼠血清瘦素、脂联素水平及脂联素受体表达水平的影响

目的 研究咖啡豆提取物对高脂饮食肥胖大鼠的瘦素(Leptin，LP)、脂联素(APN)及脂联素受体表达的影响。方法 给予高脂饲料建立SD大鼠肥胖模型，灌胃给予咖啡豆提取物(800，267，133 mg·kg^-1)，连续6周。测量大鼠体质量及脂体比，测定大鼠血浆总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、血清LP、APN；检测肝组织脂联素受体表达。结果 与正常对照组相比，给予高脂饲料6周的大鼠体质量明显增加(P<0.01)，提示SD大鼠肥胖模型建立。与模型组比较，咖啡豆提取物给药后大鼠体质量、脂体比和LP水平下降(P<0.01或P<0.05)，血清APN水平、APN受体的mRNA和蛋白表达显著增加(P<0.01或P<0.05)。结论 咖啡豆提取物对高脂饮食肥胖大鼠的体质量具有改善作用，作用机制可能是升高血清APN水平，降低LP水平及上调肝脏脂联素受体的mRNA和蛋白表达。

Effects of Coffee Bean Extracts on Serum Leptin, Adiponectin and the Expression of Adiponectin Receptors in Obese Rats

OBJECTIVE To explore the effects of coffee bean extracts on serum leptin(LP), adiponectin(APN) and the expression of adiponectin receptors in obese rats. METHODS Obese rat model was established by high fat diet. Rats obtained intragastric administration of coffee bean extracts(800, 267, 133 mg·kg^-1). The levels of plasma total cholesterol(TG), triplyceride(TC), high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C), LP, APN, weight and fat/weight were measured. The expression of adiponectin receptors in liver was measured. RESULTS Compared with normal control group, weight of the rats fed high fat diet for 6 weeks was higher(P<0.01), suggested obese rat model was established. Compared with model control group, the content of LP, weight and fat/weight of obese rats reduced after admistrated with coffee bean extracts(P<0.01 or 0.05), while APN, mRNA and protein of adiponectin receptors were significantly higher(P<0.01 or 0.05). CONCLUSION Coffee bean extracts can reduce the weight of obese rats effectively. The mechanism may be related with the reduction of the content of LP, the improvement of the content of APN and the expression of adiponectin receptors.