瓜蒌子油对糖尿病小鼠降血糖作用的研究

目的 研究瓜蒌子油对糖尿病小鼠模型的降血糖作用及其可能的作用机制。 方法 ICR小鼠尾静脉注射四氧嘧啶(100 mg/kg)造成糖尿病模型,给予不同剂量的瓜蒌子油灌胃,另设正常对照组、模型对照组和阳性药对照组,连续给药4周,每周监测小鼠体重及血糖,末次给药18 h后,摘眼球采血,分离血清,用化学比色法测血清总胆固醇(TC)、三酰甘油(TG)、一氧化氮(NO)和一氧化氮合酶(NOS)含量,用放射免疫法测血清胰岛素含量;另采用四氧嘧啶糖尿病小鼠对瓜蒌子油进行单次给药的糖耐量测定。 结果 给予瓜蒌子油不同剂量(5、10、20 ml/kg)的各组小鼠体重增长略有加快,并使血糖值呈剂量依赖性下降,能显著升高模型小鼠的血清胰岛素含量,降低血清TC、TG、NO和NOS含量;从糖耐量试验看,瓜蒌子油不同剂量组小鼠喂以淀粉后各时段血糖值呈剂量依赖性降低。 结论 瓜蒌子油具有降血糖以及改善糖耐量的作用,其降血糖的作用可能与升高血清胰岛素含量、降低血清的NO和NOS水平有关。

Decreasing effect of semen Trichosanthis oil on blood glucose on diabetic mice

Objective To study the hypoglycemic activity of semen Trichosanthis oil(STO) and the mechanism on diabetic mice. Methods The alloxan-induced diabetic mice were divided into different groups and given different dose of STO by gavage for 4 weeks, the changes of body weight and fasting blood glucose were investigated every week. Blood serum was drawn to determine TC, TG, NO, NOS and insulin. In addition, the effect of STO on glucose tolerance were also investigated in alloxan-induced diabetic mice. Results STO can dose dependently reduce the levels of blood glucose and increase the body weight when it was administrated orally at 5,10,20 ml·kg^-1, can increase insulin and lower TC,TG,NO,NOS in blood serum, and also can improve glucose tolerance in alloxan-induced diabetic mice. Conclusion STO may potentiate the hypoglycemic effect,and can improve glucose tolerance. The hypoglycemic activity of STO may be related with increasing insulin and decreasing NO and NOS in blood serum.