| 应用荧光标记PCR对散发性结直肠癌进行微卫星不稳定性检测 |
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| 引用本文： | 甘嘉亮,高枫,唐卫中,刘小健,郭云,张小龙. 应用荧光标记PCR对散发性结直肠癌进行微卫星不稳定性检测[J]. 广西医学, 2011, 33(12): 1557-1560 |
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| 作者姓名： | 甘嘉亮 高枫 唐卫中 刘小健 郭云 张小龙 |
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| 作者单位： | 广西医科大学第一附属医院结直肠肛门外科,南宁市,530021 |
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| 基金项目： | 广西科学基金资助项目(0542094) |
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| 摘 要： | 目的 检测散发性结直肠癌微卫星情况并探讨其临床意义.方法 对41例散发性结直肠癌患者,应用荧光标记PCR检测国际通用的5个微卫星位点,分析微卫星不稳定性(MSI)与临床病理参数的关系.结果 MSI检出率为24.39%(10/41),其中MSI-H为9.76%(4/41),荧光标记PCR与多重荧光PCR检出率接近;散发性...
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| 关 键 词： | 结直肠癌 微卫星不稳定 荧光标记PCR |
Detection of Microsatellite Instability in Sporadic Colorectal Cancer Using Fluorescence-labelled Primers PCR |
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| GAN Jia-liang,GAO Feng,TANG Wei-zhong,LIU Xiao-jian,GUO Yun,ZHANG Xiao-long. Detection of Microsatellite Instability in Sporadic Colorectal Cancer Using Fluorescence-labelled Primers PCR[J]. Guangxi Medical Journal, 2011, 33(12): 1557-1560 |
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| Authors: | GAN Jia-liang GAO Feng TANG Wei-zhong LIU Xiao-jian GUO Yun ZHANG Xiao-long |
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| Affiliation: | GAN Jia-liang,GAO Feng,TANG Wei-zhong,LIU Xiao-jian,GUO Yun,ZHANG Xiao-long(Department of Colorectal And Anal Surgery,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China) |
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| Abstract: | Objective To analyze the relationship between microsatellite instability and clinicopathological features in sporadic colorectal cancer and its clinical significance.Methods Fluorescence-labelled primers PCR were used to detect the microsatellite instability with global five microsatellite loci in 41 patients with sporadic colorectal cancer.The clinicopathological features characterized by microsatellite instability and microsatellite stability were compared statistically.Results The microsatellite instabil... |
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| Keywords: | Sporadic colorectal cancer Microsatellite instability Fluorescence-labelled primers PCR |
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