| HPLC法测定香青百草油中丁香酚的含量 |
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| 引用本文： | 吴运莉,舒阳,孟小夏,张敏,王鹏娇,高秀丽. HPLC法测定香青百草油中丁香酚的含量[J]. 贵阳医学院学报, 2011, 36(5): 488-490 |
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| 作者姓名： | 吴运莉 舒阳 孟小夏 张敏 王鹏娇 高秀丽 |
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| 作者单位： | 贵阳医学院药学院,贵州贵阳,550004 |
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| 摘 要： | 目的:建立一种测定香青百草油中丁香酚含量的方法.方法:采用高效液相色谱法,色谱柱Diamonsil-C18(250 mm×4.6 mm,5μm),流动相为甲醇-水(65∶35),流速为1.0 ml/min,柱温为25℃,检测波长为280 nm.结果:丁香酚在0.034～0.549 μg范围呈良好线性关系,平均回收率为9...
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| 关 键 词： | 色谱法,高压液相 香青百草油 丁香酚 |
A HPLC Method for Content Determination of Eugenol in Xiangqingbaicao Oil |
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| WU Yunli,SHU Yang,MENG Xiaoxia,ZHANG Min,WANG Pengjiao,GAO Xiuli. A HPLC Method for Content Determination of Eugenol in Xiangqingbaicao Oil[J]. Journal of Guiyang Medical College, 2011, 36(5): 488-490 |
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| Authors: | WU Yunli SHU Yang MENG Xiaoxia ZHANG Min WANG Pengjiao GAO Xiuli |
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| Affiliation: | WU Yunli,SHU Yang,MENG Xiaoxia,ZHANG Min,WANG Pengjiao,GAO Xiuli (School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China) |
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| Abstract: | Objective: To establish a method for content determination of eugenol in Xiangqingbaicao oil.Methods: The HPLC method was carried out on Diamonsil-C18(250 mm×4.6 mm,5 μm) column using methanol-water(65∶35) as mobile phase,and flow rate was 1.0ml/min.Temperature of the column was 25 ℃.Detection wavelength was 280 nm.Results: The calibration curve for eugenol was linear in the range of 0.034～0.549 μg.The average recovery was 97.63%,and RSD was 1.22%(n=9).Conclusions: The method is simple,effective,accurate and reproducible for content determination of eugenol in Xiangqingbaicao oil. |
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| Keywords: | chromatography high pressure liquid Xiangqingbaicao oil eugenol |
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