| 实时荧光定量PCR法检测糖尿病肾病大鼠肾脏中BMP-2与MGP |
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| 引用本文： | 赵安菊,黄颂敏,欧三桃,王里,陈超.实时荧光定量PCR法检测糖尿病肾病大鼠肾脏中BMP-2与MGP[J].贵阳医学院学报,2011,36(5):460-464. |
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| 作者姓名： | 赵安菊 黄颂敏 欧三桃 王里 陈超 |
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| 作者单位： | 1. 贵州省人民医院干医科,贵州贵阳,550002
2. 四川大学华西医院肾内科,四川成都,610041 |
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| 基金项目： | 贵州省科技厅基金资助项目(黔科合J字[2008]2299号); 贵州省卫生厅优秀医学青年科技人才基金项目(gzwkj2010-02-006) |
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| 摘 要： | 目的:检测糖尿病肾病(DN)大鼠肾脏中骨形成蛋白2(BMP-2)和基质Gla蛋白(MGP)的基因表达量的变化规律,探讨其对糖尿病肾病的影响.方法:设对照组(N组)及糖尿病肾病组(DN组,STZ诱导的DN大鼠模型);采用实时荧光定量PCR(Real-Time PCR)方法检测4、12、24周DN大鼠及正常大鼠肾脏中BMP...
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| 关 键 词： | 糖尿病肾病 骨形态发生蛋白质类 基质Gla蛋白 聚合酶链反应 大鼠 Sprague-Dawley |
Detection of Bone Morphogenetic Protein-2 and Matrix Gla Protein Gene Expression in Kidney of Diabetic Nephropathy Rats with Real-Time PCR |
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| ZHAO Anju,HUANG Songmin,OU Santao,WANG Li,CHEN Chao.Detection of Bone Morphogenetic Protein-2 and Matrix Gla Protein Gene Expression in Kidney of Diabetic Nephropathy Rats with Real-Time PCR[J].Journal of Guiyang Medical College,2011,36(5):460-464. |
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| Authors: | ZHAO Anju HUANG Songmin OU Santao WANG Li CHEN Chao |
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| Institution: | ZHAO Anju1,HUANG Songmin2,OU Santao2,WANG Li1,CHEN Chao1 (1.Department of Cadre's ward,the People's Hospital of Guizhou Province,Guiyang 550001,Guizhou,China,2.Department of Nephrology,Huaxi Hospital of Sichuan University,Chengdu 610041,Sichuan,China) |
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| Abstract: | Objective: To observe the expressions of bone morphogenetic protein-2(BMP-2) and matrix Gla protein(MGP)genes in kidney of diabetic nephropathy rats,and so as to explore their effects to diabetic nephropathy.Methods: Adult Sprague-Dawley male rats were divided into diabetic nephropathy group(group DN) and normal group(group N).DN model was established by STZ induction.The expressions of BMP-2 and matrix Gla protein MGP gene were detected with Real-Time PCR in 4,12 and 24 weeks.Calcium depositing around the ... |
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| Keywords: | diabetic nephropathies bone morphogenetic proteins matrix Gla protein polymerase chein reaction rats Sprague-Dawley |
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