| 中国大陆不同地域阴道毛滴虫品系的研究Ⅱ蛋白质组分的SDS-PAGE分析 |
| |
| 引用本文： | 袁丽杰,高兴政.中国大陆不同地域阴道毛滴虫品系的研究Ⅱ蛋白质组分的SDS-PAGE分析[J].中国病原生物学杂志,2003,16(3):147-148. |
| |
| 作者姓名： | 袁丽杰 高兴政 |
| |
| 作者单位： | 1. 华北煤炭医学院生物科学系,河北唐山,063000
2. 北京大学基础医学院寄生虫学教研室,北京,100083 |
| |
| 摘 要： | 目的 比较中国大陆不同地域 (北京、唐山、承德、九江 ) 7株阴道毛滴虫可溶性蛋白差异。 方法 应用聚丙烯酰胺凝胶电泳 (SDS- PAGE)、VDS凝胶分析仪对 7株阴道毛滴虫可溶性蛋白进行分析。 结果 SDS- PAGE分离出 18～2 4条蛋白带 ,7虫株共同蛋白带 15条。分子质量在 10 0 ku以上者有 2～ 6条 ,其余在 16～ 97ku之间。 结论 各地虫体蛋白质电泳图谱基本相似 ,但不同虫株蛋白条带数略有不同。
|
| 关 键 词： | 阴道毛滴虫 蛋白 SDS-PAGE |
| 文章编号： | 1001-6627(2003)03-0147-02 |
| 修稿时间： | 2002年8月20日 |
STUDY ON THE DIFFERENT ISOLATES OF TRICHOMONAS VAGINALIS IN THE MAINLAND OF CHINA Ⅱ. Analysis of SDS-PAGE protein patterns |
| |
| YUAN Li-jie,GAO Xing-zheng.STUDY ON THE DIFFERENT ISOLATES OF TRICHOMONAS VAGINALIS IN THE MAINLAND OF CHINA Ⅱ. Analysis of SDS-PAGE protein patterns[J].Journal of Pathogen Biology,2003,16(3):147-148. |
| |
| Authors: | YUAN Li-jie GAO Xing-zheng |
| |
| Institution: | YUAN Li-jie1,GAO Xing-zheng2 |
| |
| Abstract: | Objective To compare soluble proteins of the seven strains of Trichomonas vaginalis from the mainland of China(Beijing, Tangshan, Chengde, Jiujiang) and determine their similarities and/or differences. Methods Soluble proteins of the seven strains of T. vaginalis were analyzed by SDS-polyacrylamide gel eletrophoresis and VDS Gel Documentation Analysis System for understanding T. vaginalis biological property. Results A total of 18-24 distinct protein bands were demonstrated, 15 bands were common in seven isolates. Molecular weights (MWs) of 2-6 bands were more than 100 ku, The ranges in MWs of other proteins were 16-97 ku. Conclusion Similarity was observed in protein profile among different isolates, while total protein bands were of difference. |
| |
| Keywords: | Trichomonas vaginalis protein SDS-PAGE |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
