| H5N1流感疫苗株在Vero细胞中的研究[英文] |
| |
| 引用本文： | 鹿文葆,罗德炎,杨鹏辉,刘坤,刑丽,王希良,刘秀梵. H5N1流感疫苗株在Vero细胞中的研究[英文][J]. 免疫学杂志, 2010, 0(4) |
| |
| 作者姓名： | 鹿文葆 罗德炎 杨鹏辉 刘坤 刑丽 王希良 刘秀梵 |
| |
| 作者单位： | 军事医学科学院微生物流行病学研究所;病原微生物生物安全国家重点实验室;扬州大学农业部畜禽传染病学重点实验室; |
| |
| 基金项目： | supported by grants(2006AA02A204)from the National“863”great item~~ |
| |
| 摘 要： | 目的产生在Vero(非洲绿猴肾)细胞中能高适应生长的重配H5N1流感病毒疫苗株,并对其生物学特性进行初步测定。方法修饰A/Puerto Rico/8/34(H1N1)(PR8)的NS基因为Vero细胞适应型,并合成NS基因片段,构建质粒pHW2000-NS;通过RT-PCR方法,扩增疫苗株A/Anhui/01/2005(H5N1)的HA、NA基因片段,构建质粒pHW2000-HA、pHW2000-NA;以PR8的其它5个内部基因作为骨架,按照1+2+5模式8质粒共转染Vero细胞拯救流感病毒,并对拯救病毒的生物学特性进行检测。结果由Vero拯救出具有高适应生长特性的H5N1亚型人禽流感病毒疫苗株,并检测了重配疫苗株的生物学特性。结论成功从Vero细胞拯救了具有Vero细胞高适应性生长特性的H5N1亚型人禽流感病毒疫苗株,为应用Vero细胞大规模生产流感疫苗奠定了基础。
|
| 关 键 词： | H5N1流感疫苗 反向遗传学 Vero细胞 |
Development of prepared H5N1 influenza vaccine strain in Vero cells |
| |
| LU Wenbao,LUO Deyan,YANG Penghui,LIU Kun,XING Li,WANG Xiliang,LIU Xiufan State Key Laboratory of Pathogen , Biosecurity. Development of prepared H5N1 influenza vaccine strain in Vero cells[J]. Immunological Journal, 2010, 0(4) |
| |
| Authors: | LU Wenbao LUO Deyan YANG Penghui LIU Kun XING Li WANG Xiliang LIU Xiufan State Key Laboratory of Pathogen Biosecurity |
| |
| Affiliation: | LU Wenbao,LUO Deyan,YANG Penghui,LIU Kun,XING Li,WANG Xiliang,LIU Xiufan State Key Laboratory of Pathogen , Biosecurity,Institute of Microbiology , Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China |
| |
| Abstract: | The aim was to generate an attenuated and high-yielding H5N1 HPAI virus candidate vaccine from African green monkey kidney(Vero) cells by reverse genetics and to analyze its phenotype characters.The NS gene of A/Puerto Rico/8/34(H1N1) (PR8) was modified with the aim of Vero-adapting,and then synthesized to construct plasmid pHW2000;hemagglutinin(HA) and neuramindase(NA) genes of A/Anhui/01/2005(H5N1) amplified by RT-PCR were cloned into the plasmid pHW2000 for constructing pHW2000-HA and pHW2000-NA.The thre... |
| |
| Keywords: | H5N1 influenza vaccine Reverse genetics Vero cells |
| 本文献已被 CNKI 等数据库收录！ |
|
