| 幽门螺杆菌对克拉霉素耐药的分子机制研究 |
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| 引用本文： | 史彤,刘文忠,萧树东,徐蔚文. 幽门螺杆菌对克拉霉素耐药的分子机制研究[J]. 中华消化杂志, 2001, 21(1): 25-27 |
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| 作者姓名： | 史彤 刘文忠 萧树东 徐蔚文 |
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| 作者单位： | 上海第二医科大学仁济医院消化科,上海市消化疾病研究所, |
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| 基金项目： | 上海市科委资助课题(99HX002) |
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| 摘 要： | 目的：研究幽门螺杆菌（Hp)对克拉霉素耐的分子机制。方法：用E－test进行克拉霉素药敏试验，选取治疗前敏感、治疗后耐药的配对菌株及原发耐药Hp菌株进行研究；应用随机扩增多态性DNA（RAPD）分析，确定治疗前后菌株的同一性；用PCR－限制性片段长度多态性（RFLP）分析探讨克拉霉素耐药机制。结果9株克拉霉素耐药菌23SrRNA基因功能区V PCR扩增片段，8株被BsaI酶切，9株均未被BbsI酶切，提示8株在2144位点有A→G突变。结论上海地区大多数克拉霉素耐药Hp菌株存在23SrRNA基因功能区V2144位点A→G突变。
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| 关 键 词： | 幽门螺杆菌 克拉霉素 耐药 分子机制 |
| 修稿时间： | 2000-03-03 |
Molecular mechanism of Helicobacter pylori resistance to clarithromycin |
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| SHI Tong,LIU Wenzhong,XIAO Shudong,et al.. Molecular mechanism of Helicobacter pylori resistance to clarithromycin[J]. Chinese Journal of Digestion, 2001, 21(1): 25-27 |
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| Authors: | SHI Tong LIU Wenzhong XIAO Shudong et al. |
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| Affiliation: | SHI Tong,LIU Wenzhong,XIAO Shudong,et al. Shanghai Second Medical University,Renji Hospital,Shanghai Institute of Digestive Disease,Shanghai 200001,China |
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| Abstract: | Objective To investigate the molecular mechanism of Helicobacter pylori (H.pylori) resistance to clarithromycin. Methods The E test was used to determine clarithromycin resistant strains of H.pylori , and PCR Restriction Fragment Length Polymorphism (RFLP) analysis for 23S rRNA domain V gene mutations. Results Of nine clarithromycin resistant stains of H.pylori , including six primary and three acquired resistant strains, eight were found to have an A to G mutation in 23S rRNA domain V at position 2144. Conclusions The results indicated that the majority (88.8%) of clarithromycin resistant isolates of H.pylori in Shanghai have an A2144G mutation in 23S rRNA domain V. |
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| Keywords: | Helicobacter pylori Clarithromycin resistance MutatiH |
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