雄性激素对大鼠肾缺血损伤一氧化氮合酶活性的影响

目的：研究雄性激素对大鼠肾缺血损伤时肾一氧化氮合酶（nitric oxide synthase，NOS）活性及肾功能的影响。方法：应用尼克酰胺腺嘌呤二核苷酸（NADPH）-黄递酶组织化学染色法观察对照组和去势组在肾缺血损伤后NOS活性，以生物医学真彩色图象分析系统对NOS进行定量分析，同时采血检测血浆中的尿素氮（BUN）、肌苷（Cr）的变化。结果：在肾组织中NOS染色颗粒主要分布于肾小管及致密斑，肾小球着色较浅。去势组致密斑组织中NOS活性明显降低（P〈0．05），而肾小球、肾小管组织中其活性表达量差异无统计学意义（P〉0．05）。与对照组相比，去势组BUN和Cr在第0，1，7天差异无统计学意义，在第3天去势组显著升高（P〈0．05）。结论：雄激素可通过参与调节肾脏致密斑NOS活性的变化影响肾脏功能。

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Objective To investigate the effect of androgen on nitric oxide synthase activity and kidney function in the kidney of rats with ischemic injury.Methods Fourty-eight adult healthy male SD rats were randomly divided into the renal ischemia group(n=24) and the orchidectomized group with renal ischemia(n=24).The two groups were randomly divided into 4 groups by the survival time: 0,1,3 and 7 days.NADPH diaphorase histochemistry was used to measure the nitric oxide synthase activity of the kidney with ischemic injury in the orchidectomized group and the control group.The image analysis technology system was used to quantify the NOS.BUN and BCr in the plasma were detected when the rats were executed.Results The NOS histochemical staining of the kidney was mainly distributed in the renal tubule,macula densa and low in the renal glomerular.The NOS activity of macula densa significantly decreased in the orchidectomized group compared with that in the control group(P<0.05).BUN and Bcr have no significant difference on 0,1 and 7 days(P>0.05),but on the third day the data markedly increased(P<0.05).Conclusion Androgen can adjust the blood flux of glomerular by regulating the NOS activity of macula densa,and affect the kidney function.