含生物素化脂膜超声造影剂的制备与初步评价

目的 制备一种含生物素化脂膜的超声造影剂,并对其功能进行初步评价.方法 以冷冻干燥法在自制＂脂氟显＂（对照微泡）的基础上制备含生物素化脂膜超声造影剂微泡,检测其理化性质和造影功能,同时应用激光共聚焦显微镜和平行板流动腔法观察含生物素化脂膜微泡与链亲和素的黏附性.结果 ①含生物素化脂膜微泡造影剂在理化性质与小鼠肝脏造影显像方面能力与对照微泡比较差异无统计学意义（P〉0.05）;②与异硫氰酸荧光素（FITC）标记的链亲和素孵育后,含生物素化脂膜微泡荧光检测呈阳性,对照微泡为阴性;③含生物素化脂膜微泡可结合于链亲和素包被的培养皿上,且随着链亲和素包被浓度的增加其结合稳定性也相应提高.结论 应用冷冻干燥法在制备＂脂氟显＂微泡造影剂的基础上,可成功制备含生物素化脂膜超声微泡造影剂,为今后制备靶向显影及载药（基因）超声造影剂奠定了基础.

Preparation and primary evaluation of lipid-encapsulated ultrasound contrast agent with biotin PEG

Objective To prepare lipid-shelled microbubbles with biotin PEG, and to investigate their function. Methods The lipid-shelled microbubbles with biotin PEG were prepared by cryochem and machine vibration. Physicochemical properties were detected, and contrast- enhanced ultrasonography was performed. The binding abilities of microbubbles to streptavidin were assessed by eonfoeal laser scanning microscope and a parallel plate flow chamber. Results （!）There was no significant difference of physicochemical properties and echo intensity of rats liver parenchyma between lipid- shelled mierobubbles with biotin PEG and control microbubbles （ P〉0.05 ）. （~）After incubated with FITC-avidin, the mierobubbles with biotin PEG showed positive fluorescence, and the control microbubbles showed negative fluorescence. （~The binding ability increased at higher density of streptavidin. Conclusion The lipid- shelled microbubbles with biotin PEG were prepared successfully, it provides an important platform for development of targeted microbubbles.