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Methodology of using vaccinia virus to express foreign genes in tissue culture |
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| Authors: | Marion E. Perkus Elizabeth B. Kauffman Jill Taylor Susan Mercer Darlene Smith Johanna VanderHoeven Enzo Paoletti |
| Affiliation: | (1) Rensselaer Technology Park, Virogenetics Corporation, 465 Jordan Road, 12180 Troy, New York, USA |
| Abstract: | Summary A basic technique is described for inserting any foreign gene into poxvirus by in vitro recombination. Also described is a method for identifying and plaque-purifying recombinant poxvirus containing the foreign gene using nitrocellulose filters and DNA hybridization. Immunologic techniques are presented for analyzing expression of the foreign gene, either on the surface membrane or inside infected cells. |
| Keywords: | vaccinia virus orthopox virus recombination gene expression |
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