白藜芦醇对裸鼠U87细胞移植瘤生长及血管生成的影响

目的 研究白藜芦醇(Res)对人脑胶质瘤系U87细胞的体内抗癌活性及血管生成的影响. 方法 BALB/c裸鼠20只,背部皮下接种胶质瘤细胞U87建立皮下移植瘤模型.随机数字表法分为10、100mg/kgRes治疗组,溶剂对照组,空白对照组,每组5只.观察4组裸鼠移植瘤的生长曲线并应用免疫组织化学法检测瘤组织中微血管密度(MVD)及血管内皮生长因子(VEGF)的表达:采用原位末端标记法(TUNEL)检测瘤组织细胞的凋亡. 结果与空白对照组及溶剂对照组相比,100 mg/kg Res治疗组裸鼠移植瘤的体积、重量降低;100 mg/kg Res治疗组瘤组织中MVD、VEGF的表达明显降低,凋亡细胞数增高,差异均有统计学意义(P<0.05). 结论 Res对U87人脑胶质瘤细胞裸鼠移植瘤的生长具有明显抑制作用,这可能与Res导致U87移植瘤细胞凋亡及血管生成减少有关.

Effect of resveratrol on angiogenesis and growth of U87 human glioma xenografts in nude mice

Objective To explore the anti-tumor effect of resveratrol (Res) against U87 human glioma cells in nude mice and its effect on tumor angiogenesis. Methods U87 human glioma cells were implanted into the right flank of 20 nude mice, which were subsequently randomized into 4 groups (n=5), namely 10 and 100 mg/kg Res-treated group, vehicle-treated group and blank control group. The growth curve of the xenografts in the nude mice was observed and the microvessei density (MVD) and vascular endothelial growth factor (VEGF) expression in the tumor tissues were detected using immunohistochemistry. The apoptosis of the tumor cells was evaluated with TUNEL assay. Results At the daily dose of 100 mg/kg, Res significantly reduced the tumor volume and weight, and lowered the MVD and VEGF expressions as compared with the blank control and vehicle-treated groups (P<0.05), resulting also in significant U87 cell apoptosis in the tumor-bearing mice (P<0.05). Conclusion Resveratrol can significantly inhibit the growth of U87 human glioma cells implanted in nude mice possibly by inducing tumor cell apoptosis and reducing the tumor angiogenesis.