| 我国戊型肝炎病毒分离株蛋白组份分析 |
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| 引用本文： | 苑锡同 魏君. 我国戊型肝炎病毒分离株蛋白组份分析[J]. 解放军医学杂志, 1991, 16(6): 407-410 |
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| 作者姓名： | 苑锡同 魏君 |
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| 作者单位： | 军事医学科学院微生物流行病研究所(苑锡同，黄如统，许万平，魏君，黄祥瑞)，军事医学科学院微生物流行病研究所(李德荣) |
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| 基金项目： | 国家自然科学基金委员会资助 |
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| 摘 要： |
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| 关 键 词： | 戊型肝炎 病毒 蛋白组份分析 |
PROTEIN ANALYSIS OF A VIRUS ISOLATED FROM THE PATIENT''''S FECES WITH HEPATITIS E IN CHINA |
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| Yuan Xitong,Huang Rutong,Xu Wanping,Wei Jun,Huang Xiangrui,Li Derong. PROTEIN ANALYSIS OF A VIRUS ISOLATED FROM THE PATIENT''''S FECES WITH HEPATITIS E IN CHINA[J]. Medical Journal of Chinese People's Liberation Army, 1991, 16(6): 407-410 |
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| Authors: | Yuan Xitong Huang Rutong Xu Wanping Wei Jun Huang Xiangrui Li Derong |
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| Affiliation: | Yuan Xitong,Huang Rutong,Xu Wanping,Wei Jun,Huang Xiangrui,Li Derong.Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing |
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| Abstract: | A strain of 87A virus was isolated from fecal extract of a patient with epidemic enterically transmitted non-A, non-B hepatitis. It has been identified as hepatitis E virus (HEV) after a series of appraisements. The virus was inoculated into 2BS cell. The suspension of cultured virus was purified by PEG-6000 precipitation and by 10-40% sucrose density gradient centrifugation. Purified virus particles were analysed by SDS-PAGE and Western blotting with patient's sera obtained from Xinjiang and Beijing. Two regions of specific proteins have been detected. One was the region of 70-80 kD in MW, and the other in 30-40 kD. The results indicate that the strain of 87A virus is probably the Calicivirus. |
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| Keywords: | HEV Western blotting Protein analysis |
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