The Effect of Erythropoietin on Colonial Growth of Erythroid Precursor Cells In Vitro |
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| Authors: | Marvin C. Cooper Joseph Levy Linda N. Cantor Paul A. Marks Richard A. Rifkind |
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| Affiliation: | Department of Human Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, N.Y. 10032;Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, N.Y. 10032 |
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| Abstract: | A method is described for the colonial growth, in semi-solid medium, of erythropoietin-responsive erythroid cell precursors. The erythroid cell precursors were isolated by immune hemolysis from fetal mouse liver. Both the number of precursor cells triggered to proliferate and differentiate, and the size of the erythropoietic colonies formed, are directly dependent upon the concentration of erythropoietin included in the culture. |
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