犬骨骼肌卫星细胞的分离,培养及扩增方法探索

为骨骼肌卫星细胞自体移植治疗心肌疾病准备细胞材料，建立了体外分离、纯化、培养、扩增骨岂卫星细胞的方法。用机械法酶的消化法分离犬骨骼肌卫星细胞，在ｅｒｃｏｌｌ非连续密度梯度离心纯化过程后，以Ｄｅｓｍｉｎ链霉一物素一过氧化酶银川市细胞化学染色鉴定。以完全培养扩增卫星细胞。提取的卫星细胞工在９０％以中，且证明促肝细胞生长素能增强细胞的增殖能力。表明此法可获得沆纯度及治疗所需细胞数量。

The Isolation, Culture and Multiplication of Skeletal Muscle Satellite Cells

The authors had isolated skeletal muscle satellite cells by mechanic and enzymic digestion, purified them by Pecoll discontinuous density gradient centrifugation and identified them immunocytochemically by Desmin labeling,and multiplied the satellite cells by administration of promoting heptocyte growth factor (pHGF). The purity of harvested satellite cells exceeded 90% .It was suggested that the pHGF was usful for prompting satellite cell growth by shortening the lag period and enhancing their proliferation capacities.